骨碎补总黄酮对卵巢去势大鼠骨质疏松的干预作用

Intervention effect of Rhizoma Drynariae Total Flavone on osteoporosis in ovariectomized rats

目的探讨骨碎补总黄酮对大鼠骨质疏松(OP)的干预作用。方法选取21只斯普拉格-杜勒(SD)大鼠,按照随机数字表法将其分为空白组、模型组、治疗组,每组各7只。空白组不做处理,模型组制备卵巢摘除诱导OP模型,治疗组造模后给予0.054 g/(kg·d)骨碎补总黄酮灌胃。应用双能X线骨密度仪(DXA)检测右侧股骨头骨密度(BMD)。通过酶联免疫吸附试验(ELISA)检测血清中骨转化标志物骨钙素(BGP)、骨碱性磷酸酶(BALP)、甲状腺素(PTH)水平,镜下观察骨组织苏木素-伊红(HE)染色病理学变化。结果干预前,模型组的BMD低于空白组,差异有统计学意义(P<0.05);治疗组的BMD与模型组比较,差异无统计学意义(P>0.05)。干预后,空白组和治疗组的BMD高于模型组,差异有统计学意义(P<0.05);治疗组的BMD低于空白组,差异有统计学意义(P<0.05)。三组干预前后组内的BMD比较,差异均无统计学意义(P>0.05)。镜下观察见治疗组股骨组织骨小梁数量、密度、布局和完整性均好于模型组。ELISA结果显示,模型组的BGP低于空白组,BALP、PTH高于空白组,差异有统计学意义(P<0.05);治疗组的BGP低于空白组,BALP、PTH高于空白组,差异有统计学意义(P<0.05);治疗组的BGP高于模型组,BALP、PTH低于模型组,差异有统计学意义(P<0.05)。结论对去势诱导的OP给予骨碎补总黄酮干预具有良好的改善作用。

Objective To explore the intervention effect of Rhizoma Drynariae Total Flavone on osteoporosis(OP)in rats.Methods A total of 21 Sprague-Dulle(SD)rats were selected and divided into blank group,model group and treatment group according to the random number table method,with 7 rats in each group.The blank group was left untreated,ovariectomy-induced osteoporosis model was established in the model group,and the treatment group was administered with 0.054 g/(kg·d)Rhizoma Drynariae Total Flavone after model establishment.The bone mineral density(BMD)of the right femur was measured by the dual-energy X-ray absorptiometry.The expression levels of bone transformation markers osteocalcin(BGP),the bone alkaline phosphatase(BALP)and the thyroxine(PTH)in serum were detected by the enzyme-linked immunosorbent assay(ELISA),and the histopathological changes of bone hematoxylin-eosin(HE)staining were observed by microscope.Results Before the intervention,the BMD of the model group was lower than that of the blank group,with statistically significant difference(P<0.05).There was no significant difference in BMD between the treatment group and the model group before the intervention(P>0.05).After the intervention,the BMD of the blank group and the treatment group was higher than that of the model group,with statistically significant difference(P<0.05).The BMD in the treatment group was lower than that in the blank group after the intervention,and the difference was statistically sigdifference in BMD among the three groups before and after the intervention(P>0.05).The HE staining showed that the number,density,layout and integrity of trabeculae in the femoral bone tissue of the treatment group were better than those of the model group.The ELISA results showed that the BGP in the model group was lower than that in the blank group,BALP and PTH were higher than those in the blank group,and the differences were statistically significant(P<0.05).The BGP of the treatment group was lower than that of the blank group,BALP and PTH were higher than those of the blank group,and the differences were statistically significant(P<0.05).The BGP of the treatment group was higher than that of the model group,BALP and PTH were lower than those of the model group,and the differences were statistically significant(P<0.05).Conclusion The Rhizoma Drynariae Total Flavone intervention has a good effect on the OP induced by castration.