螺旋藻多糖对急性T淋巴细胞白血病细胞的杀伤作用

Cytotoxic effects of spiruline platensis polysaccharides on acute T lymphocyte leukemia

目的研究螺旋藻多糖(SPP)对人急性T淋巴细胞白血病细胞系Jurkat的杀伤作用。方法取对数生长期Jurkat细胞,分为对照组和1 g/L、2 g/L、5 g/L SPP组。采用MTT法检测SPP对Jurkat细胞增殖的影响,Western blot法检测凋亡蛋白caspase-3、细胞膜表面受体CD45、CD71以及细胞内部信号分子p-ERK、p-JNK、t-ERK和t-JNK蛋白表达水平。结果1 g/L、2 g/L和5 g/L SPP组细胞增殖抑制率依次升高,分别为53.286%±3.112%、80.075%±4.240%和86.430%±4.614%。2 g/L、5 g/L SPP组cleaved caspase-3蛋白表达水平高于对照组,5 g/L SPP组高于1 g/L SPP组(P<0.05)。1 g/L、2 g/L SPP组CD45蛋白表达水平高于对照组(P<0.05),CD71蛋白表达水平与对照组差异无统计学意义;5 g/L SPP组CD45蛋白表达水平均高于对照组、1 g/L和2 g/L SPP组;CD71蛋白表达水平低于对照组(P<0.05),与1 g/L和2 g/L SPP组差异无统计学意义。对照组、1 g/L SPP组、2 g/L SPP组和5 g/L SPP组p-ERK蛋白表达水平依次升高,组间多重比较差异均有统计学意义;各组间t-ERK、p-JNK和t-JNK蛋白表达水平差异均无统计学意义。结论SPP对Jurkat细胞具有杀伤作用,其机制可能是SPP通过影响细胞膜表面受体CD45和CD71的表达,将凋亡信号传递到细胞内部,通过活化ERK,最终活化caspase-3,诱导细胞凋亡。

Objective To investigate the cytotoxic effect of spirulina platensis polysaccharides(SPP)on acute T lymphocyte leukemia Jurkat cells.Methods SPP was obtained by water extraction and ethanol precipitation.The effect of SPP on cell proliferation was determined by MTT assay.Caspase-3 was tested by Western blot assay.Furthermore,the cell surface expression of CD45 and CD71,and the expression levels of p-ERK,p-JNK,t-ERK and t-JNK were also evaluated.Results SPP inhibited Jurkat cell proliferation in a dose-dependent manner(1 g/L,2 g/L and 5 g/L),the inhibitory rates were 53.286%±3.112%,80.075%±4.240%and 86.430%±4.614%,respectively.The expression levels of cleaved caspase-3 were higher in 2 g/L and 5 g/L SPP groups than those in the control group,and that in 5 g/L SPP group was higher than that in 1 g/L SPP group(P<0.05).The expression levels of CD45 were higher in 1 g/L and 2 g/L SPP groups than those in the control group(P<0.05),while there was no significant difference in the expression level of CD71 between the two groups.The expression levels of CD45 were higher in 5 g/L SPP group than those in control group,1 g/L SPP group and 2 g/L SPP group.The expression level of CD71 was lower in 5 g/L SPP group than that in control group(P<0.05),and there was no significant difference in the expression level of CD71 between 1 g/L and 2 g/L SPP groups.The expression levels of p-ERK were increased significantly in turn in the control group,1 g/L,2 g/L and 5 g/L SPP groups.There were no significant differences in expression levels of t-ERK,p-JNK and t-JNK between groups.Conclusion SPP could inhibit Jurkat cell growth and induce cell death,which might through affecting CD45 and CD71 expression on cell surface,then activating ERK and finally activating caspase-3 to induce cell apoptosis.