摘要
基于时间分辨荧光微球制备的呕吐毒素荧光定量快速检测试纸卡,通过两线及三线试纸卡灵敏度与重复性的对比,筛选出效果更优的三线试纸卡,并对三线试纸卡不同性能要求进行检测验证。结果表明,呕吐毒素三线试纸卡检出限与定量限符合要求,准确度、重复性、稳定性等均达到更优的效果,更能满足快速定量检测要求。
The quantitative&rapid test strip for Deoxynivalenolis prepared based on the time-resolved fluorescence microsphere.Through the comparison of sensitivity and repeatability of two-line and three-line strip,the better three-line strip was selected,and the different performance requirements of the three-line strip were tested and verified.The results showed that the detection limit and quantitative limit of Deoxynivalenol three-line strip met the requirements,and the accuracy,repeatability and stability of the strip were better,which could meet the requirements of rapid quantitative detection.
作者
巩性涛
王培
肖理文
宋永泉
徐秀
赵皖
王春霞
GONG Xingtao;WANG Pei;XIAO Liwen;SONG Yongquan;XU Xiu;ZHAO Wan;WANG Chunxia(Shandong Grain&Oil Testing Centerjinan 250101,China;Nanjing Micro-Testing Biology Science Technology Co.,Ltd,Nanjing210000,China;Shandong Grain and Oil Quality Supervision Center Co.Ltd.Jinan 250199,China)
出处
《粮食加工》
2021年第2期84-91,共8页
Grain Processing
基金
山东省2019年重点研发计划项目(2019GSF111012)
关键词
呕吐毒素
三线试纸卡
时间分辨荧光免疫层析法
检出限
定量限
准确性
重复性
Deoxynivalenol
three line test paper strip
time resolved fluorescence immunochromatography
detection limit
limit of quantification
accuracy
repeatability