不同超声波处理方式辅助三相分配法提取琉璃苣籽油的比较

Comparison of different ultrasound treatments assisted three phase partitioning for extraction of Borago officinalis seed oil

采用三相分配法(TPP)提取琉璃苣籽油,以琉璃苣籽油得率为指标,对提取时间、料液比、硫酸铵加量、水相与叔丁醇体积比进行了研究;同时比较了超声波预处理辅助三相分配法(UPTPP)和超声波辅助三相分配法(UTPP)对琉璃苣籽油提取效果的影响。结果表明:采用TPP,在料液比1∶15、提取时间20 min、硫酸铵加量0.35 g/mL、水相与叔丁醇体积比1∶2时,琉璃苣籽油得率最大,为31.26%;采用超声波作为过程强化方法,可缩短TPP的提取时间,且UPTPP优于UTPP,在超声波预处理时间160 s、超声功率100 W、占空比50%时,琉璃苣籽油得率为33.57%,比TPP提高了2.31个百分点。因此,超声波预处理后采用三相分配法是一种有效的油脂提取方法。

The Borago officinalis seed oil was extracted using three phase partitioning (TPP). The effects of various factors including extraction time,solid-liquid ratio,dosage of ammonium sulfate and volume ratio of aqueous phase to t-butanol on the yield of Borago officinalis seed oil were studied,and these parameters were optimized. Moreover,the effects of ultrasound pretreatment assisted TPP and ultrasound assisted TPP on the extraction of Borago officinalis seed oil were compared. The results showed that the optimal conditions of TPP were obtained as follows: solid-liquid ratio 1∶15,extraction time 20 min,dosage of ammonium sulfate 0. 35 g/mL and volume ratio of aqueous phase to t-butanol 1∶2. Under these conditions,the yield of Borago officinalis seed oil was 31. 26%. When ultrasound was used as process intensification method,the extraction time could be shortened,and the ultrasound pretreatment assisted TPP process was better than ultrasound assisted TPP process. Under the conditions of ultrasound pretreatment time 160s,ultrasound power 100 W and duty cycle 50%,the yield of Borago officinalis seed oil was 33. 57%,which was 2. 31 percentage points higher than that of TPP. So,TPP after ultrasound pretreatment was an effective method for oil extraction.