基于UPLC/Q-TOF MS/MS的花生油成分分析

Component analysis of peanut oil based on UPLC/Q-TOF MS/MS

采用超高效液相色谱-串联四级杆飞行时间质谱(UPLC/Q-TOF MS/MS)联用仪对花生油甲醇提取液分离检测,分析花生油成分,并通过二级质谱图和标准品确定物质。结果表明:正离子模式下检出86种化合物,鉴定30种物质,其中芥酸、棕榈酰胺、硬酯酰胺、油酸酰胺、11Z-二十二碳二烯酸为已知的花生油成分,亚油酰乙醇胺、亚油酰胺、鞘氨醇、N-油酰乙醇胺、十八碳四烯酸、顺-4-癸二酸等26种物质均为首次被检出;在负离子模式下,共检出27种化合物,鉴定15种物质,其中包括花生油中常见的7种脂肪酸,首次检出(Z)-13-氧-9-十八烯酸、蓖麻油酸、烯油酸、13-羟基十八酸和2(R)-羟基海藻酸5种脂肪酸和其他3种物质。

The methanol extract of peanut oil was detected by ultra performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS/MS) to analyze the component in peanut oil,and using the secondary mass spectrometry and standard products were used to determine the component. The results showed that in positive ion mode,86 compounds were detected,and 30 compounds were identified. Among them,erucic acid,palmitamide,stearamide,oleamide and 11Z-docosadienoic acid were the known components in peanut oil,and other 26 substances including linoleylethanolamine,linoleamide,sphingosine,N-oleylethanolamine,octacosatetraenoic acid,and cis-4-sebacic acid were detected for the first time. In negative ion mode,27 compounds were detected and 15 compounds were identified in peanut oil,including 7 common fatty acids. Five fatty acids including (Z)-13-oxy-9-octadecenoic acid,ricinoleic acid,limonenic acid,13-hydroxyoctadecanoic acid and 2 (R)-hydroxyalginic acid and 3 other substances were first detected in peanuts oil.