葛根素对大鼠乳房脂肪组织增殖的影响及其机制研究

Effects of Puerarin on Rat Breast Adipose Tissue Proliferation and its Mechanism

目的:通过研究葛根素(Puerarin,Pur)对3T3-L1前脂肪细胞增殖分化及大鼠乳房脂肪组织的影响及其相关蛋白表达的调控,旨在探讨Pur对乳房脂肪组织的影响及其机制。方法:采用体外培养3T3-L1前脂肪细胞测定不同浓度Pur对3T3-L1脂肪细胞分化过程中胞浆脂滴积累的影响,通过Western blot、免疫荧光实验检测Pur对解偶联蛋白(Uncoupling protein 1,UCP-1)、重组人CCAAT增强子结合蛋白(CCAA Tenhancer-binding proteinα,C/EBP-α)表达水平;进一步考察大鼠乳房涂抹不同浓度的Pur对脂肪组织增殖的影响,通过HE染色检测乳腺脂肪细胞体积、免疫组化检测乳腺脂肪蛋白,分析其增殖机制。结果:Pur通过上调3T3-L1细胞中脂肪分化相关蛋白UCP-1、C/EBPα的表达,显著促进3T3-L1脂肪细胞分化过程中胞浆脂滴积累。此外,体内实验结果显示Pur能促进大鼠乳房脂肪细胞增多,脂肪细胞直径增大,且促进乳腺UCP-1、C/EBPα的表达。结论:Pur显著促进大鼠乳房脂肪组织增殖,其作用机制可能与上调脂肪分化相关的蛋白UCP-1、C/EBPα的表达有关。

Objective To study the effect of puerarin(Pur)on the proliferation and differentiation of 3T3-L1 preadipocytes and rat breast adipose tissue and the regulation of related protein expression,aiming to explore the effect of puerarin on breast adipose tissue And its mechanism.Methods 3T3-L1 preadipocytes were cultured in vitro to determine the effect of different concentrations of puerarin on the accumulation of cytoplasmic lipid droplets during the differentiation of 3T3-L1 adipocytes.The expression level of the uncoupling protein and the recombinant human CCAAT enhancer binding protein(C/EBP-α)was detected by Western blot and immunofluorescence experiments.Further,to investigate the effect of applying different concentrations of puerarin on the proliferation of adipose tissue in rat breasts,The volume of mammary fat cells was detected by HE staining,and the mammary adipose protein was detected by immunohistochemistry,and the proliferation mechanism was analyzed.Results Pur up-regulated the expression of UCP-1 and C/EBPαin 3T3-L1 cells,which significantly promoted the accumulation of cytoplasmic lipid droplets during the differentiation of 3T3-L1 cells.In addition,in vivo experiments show that Pur can promote the increase of rat breast fat cells,increase the diameter of fat cells,and promote the expression of breast UCP-1 and C/EBPα.Conclusion Pur significantly promotes the proliferation of rat breast adipose tissue,and its mechanism may be related to the up-regulation of the expression of UCP-1 and C/EBPα,which are related to fat differentiation.