E盒结合锌指蛋白2与结肠癌发生发展及预后的关系

Relationship between ZEB2 Protein and Development and Prognosis of Colon Cancer

【目的】探讨E盒结合锌指蛋白2(ZEB2)与结肠癌发生发展及预后的关系。【方法】选取本院病理中心收集的90例结肠癌手术后病理学标本、选取45例符合要求的结肠癌癌旁组织标本作为对照组;采用Westernblot技术检测两组标本中的ZEB2蛋白表达强度,分析不同年龄、性别、病灶直径、肿瘤部位、TNM分期、肿瘤分化程度、淋巴结转移情况、不同预后患者结肠癌组织中ZEB2蛋白表达差异,采用Logistic回归方法分析ZEB2蛋白与结肠癌患者预后的关系。【结果】结肠癌组织中的ZEB2蛋白表达强度高于对照组,且差异有显著性(P<0.05);不同年龄、性别,不同病灶直径、不同组织分化程度、不同结肠部位的结肠癌组织中的ZEB2蛋白表达强度组间比较差异均无显著性(P>0.05);不同TNM分期、是否发生淋巴结转移、2年随访不同结局的结肠癌患者ZEB2蛋白表达强度组间比较差异均有显著性(P<0.05);logistic回归分析显示TNM分期越高、发生淋巴结转移、ZEB2蛋白表达增强是结肠癌患者不良预后的独立危险因素(P<0.05)。【结论】结肠癌组织中的ZEB2蛋白表达上调,并且与肿瘤的发生发展及与患者的预后有关。

【Objective】To investigate the relationship between E-box binding zinc finger protein 2(ZEB2)and the development and prognosis of colon cancer.【Methods】The pathological specimens of 90 colon cancer patients collected from the pathology center of our hospital were selected,and 45 specimens of adjacent colon cancer tissues were selected as the control group.The intensity of ZEB2 protein expression in the two groups was detected by Western-blot technique.The differences of ZEB2 protein expression in colon cancer tissues with different age,gender,lesion diameter,tumor location,TNM stage,tumor differentiation,lymph node metastasis and colon cancer were analyzed by logistic regression.The relationship between ZEB2 protein and prognosis of colon cancer patients was analyzed by logistic regression.【Results】The expression of ZEB2 protein in colon cancer tissues was higher than that in the control group,and the difference was statistically significant(P<0.05).ZEB2 protein in colon cancer tissues with different ages,genders,different lesion diameters,different tissue differentiation degrees and different colon sites.There was no statistically significant difference between the expression intensity groups(P>0.05).The differences in ZEB2 protein expression between the different TNM stages,lymph node metastasis,and 2-year follow-up of different outcomes were statistically significant.(P<0.05);Logistic regression analysis showed that the higher the TNM stage,the lymph node metastasis and the increased ZEB2 protein expression were independent risk factors for poor prognosis in colon cancer patients(P<0.05).【Conclusion】ZEB2 protein expression is up-regulated in colon cancer tissues and is associated with tumor development and prognosis.