速效救心丸对心肌缺血大鼠心肌损伤相关蛋白表达的影响

Effect of Suxiao Jiuxin Pill on ischemic injury-related protein expression in myocardiac tissue of rats with myocardial ischemia

目的研究速效救心丸对心肌缺血大鼠心肌损伤相关蛋白表达的影响。方法雄性Wistar大鼠随机分为对照组、模型组及速效救心丸低、中、高剂量(250、500、1000 mg/kg)组,对照组给予正常饲料,模型组与各给药组大鼠给予高脂饲料喂养24周。第24周,各给药组ig速效救心丸,1次/d,连续7 d;给药第5天,模型组与给药组大鼠ip垂体后叶素(30U/kg),1次/d,连续3 d。最后一次ip垂体后叶素后,大鼠经腹主动脉取血,ELISA法检测血清中肌酸激酶同工酶(creatine kinase isoenzyme,CK-MB)、心肌肌钙蛋白T(cardiac troponin T,c TnT)、心肌肌钙蛋白I(cardiac troponin I,c TnI)、血栓素B2(thromboxane B2,TXB2)、6-酮-前列环素F-1α(6-ketone-prostacycline F-1α,PGF-1α)和内皮素-1(endothelin 1,ET-1)水平;心脏灌流后取心脏,采用苏木素-伊红(HE)染色法考察速效救心丸对心肌缺血大鼠心脏组织病理变化的影响,采用Westernblotting法考察速效救心丸对心肌缺血大鼠心脏组织中心肌损伤相关蛋白血管内皮生长因子(vascularendothelial growth factor,VEGF)、ET-1、内皮型一氧化氮合酶(endothelial nitric oxide synthase,e NOS)、低氧诱导因子-1α(hypoxia inducing factor-1α,HIF-1α)、蛋白激酶B(proteinkinaseB,Akt)和磷酸化Akt(p-Akt)表达的影响。结果与模型组比较,速效救心丸组大鼠血清中CK-MB、c TnT、cTnI、TXB2、PGF-1α和ET-1水平均显著降低(P<0.05);心肌组织中HIF-1α、VEGF和ET-1蛋白表达水平显著降低(P<0.05),p-Akt/Akt和e NOS蛋白表达水平显著升高(P<0.05),呈剂量相关性。结论速效救心丸对心肌缺血大鼠有明显预防作用,其作用机制与调控心肌缺血损伤相关蛋白的表达密切相关。

Objective To study the effect of Suxiao Jiuxin Pill(SJP,速效救心丸)on ischemic injury-related protein expression in myocardiac tissue of rats with myocardial ischemia.Methods Male Wistar rats were randomly divided into control group,model group,low-,medium-and high-dose(250,500,1000 mg/kg)SJP groups.Rats in control group were fed with normal diet,rats in model and SJP groups were fed with high-fat diet for 24 weeks.On the 24 th week,rats in each administration group were ig SJP,once a day for 7 d;On the 5 th day of administration,rats in model group and administration group were ip pituitary gland(30 U/kg),once a day for 3 d.After the last injection,blood was collected from abdominal aorta,the levels of creatine kinase isoenzyme(CK-MB),cardiac troponin T(c Tn T),cardiac troponin I(cTnI),thromboxane B2(TXB2),6-ketone-prostacycline F-1α(PGF-1α),and endothelin 1(ET-1)in serum were detected by ELISA.After cardiac perfusion,the heart was taken,the effect of SJP on the pathological changes of cardiac tissue in rats with myocardial ischemia was observed by HE staining method.The effects of SJP on expressions of myocardial injury-related proteins such as vascular endothelial growth factor(VEGF),ET-1,inducible endothelial nitric oxide synthase(eNOS),hypoxia inducing factor-1α(HIF-1α),protein kinase B(protein kinase B,Akt),and phosphorylated Akt(p-Akt)in the cardiac tissue of myocardial ischemic rats were detected by Western blotting.Results Compared with model group,the serum levels of CK-MB,cTnT,cTnI,TXB2,PGF-1α,and ET-1 in SJP group were significantly reduced(P<0.05);The expressions of HIF-1α,VEGF,and ET-1 in the myocardial tissue were significantly reduced(P<0.05),and the expressions of p-Akt/Akt and eNOS were significantly increased(P<0.05),which showed a dose-related relationship.Conclusion SJP has a significant preventive effect on myocardial ischemia rats,of which mechanism is closely related to the regulation of the expression of key myocardial proteins related to ischemia injury.