老鸦瓣芽茎发育相关MYB转录因子家族鉴定与分析

Identification and analysis of MYB transcription factor family related to stolon development of Amana edulis

目的鉴定老鸦瓣Amana edulis MYB转录因子家族成员并分析其表达模式,以期发现老鸦瓣芽茎发育相关MYB基因。方法利用老鸦瓣转录组数据经Pfam和PlnTFDB数据库鉴定含有MYB保守结构域的MYB转录因子,通过构建系统进化树并根据拟南芥MYB分组信息为老鸦瓣R2R3-MYB基因划分亚组,进行2R-MYB结构域可视化分析和GO富集分析,挑选在芽茎中特异表达的MYB基因并用实时荧光定量PCR(qRT-PCR)验证表达。结果共鉴定出93个含有MYB保守结构域的MYB转录因子,其中53个R2R3-MYB分为15个亚组,GO注释为生物过程的子类别最多,在芽茎中特异表达的12个MYB基因中有11个基因在芽茎发育前期至中期的表达量显著下降,有4个基因在芽茎发育中期至后期的表达量显著上升。结论获得的53个R2R3-MYB转录因子为进一步研究老鸦瓣MYB转录因子家族影响芽茎发育过程的分子机制奠定了基础。

Objective The current study was carried out to identify and explore the expression pattern analysis of MYB transcription factors family in Amana edulis.Methods MYB transcription factors with conserved domain were identified by Pfam and PlnTFDB data using the transcriptome of A.edulis,the phylogenetic tree was constructed,and the subgroup of R2R3-MYB genes were classified according to the Arabidopsis thaliana grouping information with MYB.In addition,2R-MYB domains visualization analysis and GO enrichment analysis were carried out,and the specific expression of MYB genes in stolon was selected to verify expression by qPCR.Results A total of 93 MYB transcription factors with conserved domain were identified,among which 53 R2R3-MYB genes were divided into 15 subgroups.And the most subcategories of the GO annotation were biological processes.The expression of 11 of the 12 MYB genes with expressed specifically in stolon was decreased significantly from early to middle development stage,and the expression of four genes was increased significantly from middle to late development stage of stolon.Conclusion The 53 R2R3-MYB transcription factors obtained in this study provided the foundation for further study on the molecular mechanism of MYB transcription factors family in A.edulis stolon development.