摘要
To develop a biodegradable membrane with guided bone regeneration(GBR),a Mg-2.0Zn-1.0Gd alloy(wt.%,MZG)membrane with Ca-P coating was designed and fabricated in this study.The microstructure,hydrophilicity,in vitro degradation,cytotoxicity,antibacterial effect and in vivo regenerative performance for the membrane with and without Ca-P coating were evaluated.After coating,the membrane exhibited an enhance hydrophilicity and corrosion resistance,showed good in vitro cytocompatibility upon MC3T3E-1 cells,and exhibited excellent antibacterial effect against E.coli,Staphylococcus epidermis and Staphylococcus aureus,simultaneously.In vivo experiment using the rabbit calvarial defect model confirmed that Ca-P coated MZG membrane underwent progressive degradation without inflammatory reaction and significantly improved the new bone formation at both 1.5 and 3 months after the surgery.All the results strongly indicate that MZG with Ca-P coating have great potential for clinical application as GBR membranes.
基金
This work was supported by National Natural Sci-ence Foundation of China(No.81600827,No.U1804251,No.81600827 and No.51971134)
the National Key R&D program of China(No.2016YFC1102103)
the Science and Technology Commission of Shanghai(18441908000)
Shanghai Jiao Tong University Biomedi-cal Engineering Research Fund(YG2019ZDA02).Dr.Jiawen Si wants to thank his wife Qifan Hu and daughter Jinnuo Si for their support,care and love over the past years,and say“thank god for sending you to me on angel’s wings”.
