栉孔扇贝中蛋白质的提取条件优化

Optimized Extraction Conditions of Protein from Scallop Chlamys farreri

为研究栉孔扇贝蛋白质的碱法提取工艺,优化提取条件。本研究以蛋白质提取率为指标,通过pH值、温度、料液比及反应时间的单因素实验和正交实验对蛋白质提取条件进行优化,通过复溶、硫酸铵沉淀、透析、冷冻干燥得到栉孔扇贝蛋白质干品。结果表明,栉孔扇贝蛋白质的最优碱提工艺参数为pH值12、温度40℃、料液比1∶30(W/W)、时间6 h。在最优碱提条件下提取,蛋白质的提取率高达74.33%。用50%~60%饱和区间的硫酸铵溶液进行碱溶蛋白回收时,蛋白质沉淀率达到90.19%,栉孔扇贝中蛋白质的总体回收率可达到67.04%。该研究为栉孔扇贝蛋白质的提取和分离提供了借鉴,为后续栉孔扇贝蛋白质的开发与利用提供了理论依据。

To study alkali extraction technology of Chlamys farreri protein and optimize the extraction conditions,the protein extraction rate was taken as the index,and the protein extraction conditions including pH,temperature,solid-liquid ratio and reaction time were optimized by the single factor and orthogonal experiment.The dry protein of Chlamys farreri was obtained by redissolving,ammonium sulfate precipitation,dialysis and freeze-drying.The results showed that the optimal alkali extraction process parameters of Chlamys farreri protein were pH 12,temperature 40℃,ratio of solid to liquid 1∶30(W/W),and extraction time 6 h.Under the optimal alkali extraction conditions,the protein extraction rate was as high as 74.33%.When 50%to 60%saturation range of ammonium sulfate solution was used for alkali-soluble protein recovery,the protein precipitation rate reached 90.19%,and the overall recovery rate of protein in Chlamys farreri reached 67.04%.In conclusion,this study provides a reference for the extraction and separation of Chlamys farreri protein,and provides a theoretical basis for the subsequent development and utilization of Chlamys farreri protein.