安宁思茅松毛虫6龄幼虫肠道可培养细菌的多样性研究

Diversity of Intestinal Cultivable Bacteria from 6^(th) Instar Larvae of Dendrolimus kikuchii in Anning City,Yunnan Province

为给思茅松毛虫的综合防治提供参考措施,以安宁地区思茅松毛虫(Dendrolimu kikuchii)6龄幼虫为对象,采用纯培养法对其肠道细菌进行分离纯化,并进行形态特征观察及生理生化指标测定,对菌株进行初步鉴定,再结合16S rDNA分子鉴定技术进行分析,初步判定细菌的分类学地位,研究其肠道细菌的多样性。结果显示:从6龄思茅松毛虫幼虫肠道中共分离到104株细菌,初步鉴定隶属于10个属14个类群,分别为55株芽孢杆菌属(5个类群)、9株类芽孢杆菌属(1个类群)、2株苍白杆菌属(1个类群)、5株短芽孢杆菌属(1个类群)、4株微球菌属(1个类群)、6株莫拉菌属(1个类群)、8株栖水菌属(1个类群)、2株土壤芽孢杆菌属(1个类群)、7株葡萄球菌属(1个类群)、6株普罗威登斯菌属(1个类群)。其中芽孢杆菌属具有最高的相对分离率,为53.00%。6龄幼虫肠道细菌的Shannon多样性指数、Simpson优势度指数、Margalef丰富度指数分别为2.5217、0.9129、2.7991。思茅松毛虫6龄幼虫肠道细菌的多样性丰富,芽孢杆菌属为优势菌属,这些菌种为研究思茅松毛虫综合防治奠定了基础。

In order to provide reference measures for the comprehensive control of Dendrolimus kikuchii,by taking 6 th instar larvae of D.kikuchii as the object,the pure culture method was used to isolate and purify the 6 th instar larvae of D.kikuchii collected from the Anning area.The morphology of the isolated strains was observed and the physiological and biochemical index were determined.The strains were initially identified by combing with 16S rDNA molecular identification technology,their taxonomy status was preliminarily determined,and studied the diversity of intestinal bacteria.The results showed that a total of 104 strains of bacteria were isolated from 506 th instar larvae and belonged to 10 genera and 14 groups,i.e.55 strains of Bacillus sp.(5 groups),9 strains of Paenibacillus sp.(1 group),2 strains of Ochrobactrum sp.(1 group),5 strains of Brevibacillus sp.(1 group),4 strains of Micrococcus sp.(1 group),6 strains of Moraxella sp.(1 group),8 strains of Enhydrobacter sp.(1 group),2 strains of Solibacillu sp.(1 group),7 strains of Staphylococcus sp.(1 group)and 6 strains of Providencia sp.(1 group).Among them,Bacillus has the highest relative isolation rate of 53.00%.The Shanno diversity index,Simpson dominance index and Margalef richness index of intestinal culturable bacterial from 6 th instar were 2.5217,0.9129,and 2.7991,respectively.The diversity of the 6 th instar larvae of D.kikuchii are rich and the Bacillus is the dominant bacteria.These strains lay a research foundation for the comprehensive prevention of D.kikuchii.