碳纳米溶胶对烟草K^(+)通道基因表达及根系K^(+)流速的影响

Effects of carbon-nano sol on expression of K^(+)channel gene in tobacco and K^(+)fluxes in tobacco roots

为解析碳纳米溶胶促进烟草钾素吸收的生理机制,利用砂培试验和非损伤微测技术(Non-invasive micro-test technology,NMT),研究了碳纳米溶胶对4个烟草品种K^(+)通道基因表达、根系K^(+)流速、植株钾素吸收和积累的影响。结果表明:碳纳米溶胶能促进烟草K^(+)内流通道基因的表达,促进K^(+)内流,抑制K^(+)外流,且不同品种、不同部位的K^(+)通道基因对碳纳米溶胶的响应各不相同。在碳纳米溶胶处理下,RG17、鄂烟1号、云烟87和中烟100的K^(+)外流通道基因NTORK1表达量相比对照分别降低31.1%、59.9%、25.6%和32.0%;K^(+)内流通道基因NKT1在鄂烟1号和云烟87根系中的表达显著增加,增幅分别为210.6%和130.9%。碳纳米溶胶处理增加了各烟草品种根系的K^(+)流速,与对照相比,RG17、鄂烟1号、云烟87和中烟100经碳纳米溶胶处理后K^(+)内流速率分别增加38.17、39.71、13.96、8.61 pmol·cm^(-2)·s^(-1)。此外,经碳纳米溶胶处理后,鄂烟1号、云烟87和中烟100植株钾素积累量较对照分别增加了18.6%、15.6%和46.6%。可见,碳纳米溶胶通过调节根系K^(+)通道基因的表达可增强根系对钾素的吸收并促进烟草体内钾素的积累,但不同烟草品种对碳纳米溶胶的响应存在明显差异。

In order to analyze the physiological mechanism behind the promotional effects of carbon-nano sol on the potassium absorption by tobacco,sand culture experiments and non-invasive micro-test(NMT)were used to study the effects of carbon-nano sol on K^(+)channel gene expressions,root K^(+)fluxes,potassium uptakes and accumulations by four tobacco varieties,RG17,Eyan 1,Yunyan 87 and Zhongyan 100.The results showed that carbon-nano sol could promote the expression of K^(+)influx channel gene and K^(+)influxes and inhibit K^(+)effluxes however the responses of K^(+)channel gene in different varieties and different parts of tobacco were different Under the carbon-nano sol treatment,the expressions of K^(+)efflux channel gene NTORK1 in RG17,Eyan 1Yunyan 87 and Zhongyan 100 decreased by 31.1%,59.9%,25.6%and 32.0%respectively,as compared with the control.the expression of NKT1 in the roots of Eyan 1 and Yunyan 87 significantly increased by 210.6%and 130.9%respectively.Carbon-nano sol treatment raised the K^(+)fluxes of the roots of each tested leaf variety Compared with the control,the K^(+)influxes of RG17,Eyan 1,Yunyan 87 and Zhongyan 100 increased by 38.1739.71,13.96 and 8.61 pmol·cm^(-2)·s^(-1) respectively under the carbon-nano sol treatment.In addition,the potassium accumulations in Eyan 1,Yunyan 87 and Zhongyan 100 increased by 18.6%,15.6%and 46.6%respectively,as compared with the control.In conclusion,by regulating the expression of K^(+)channel gene in the root system the carbon-nano sol could enhance the uptake of potassium by the root system and promote the accumulation of potassium in tobacco.There were significant differences in the responses of different tobacco varieties to the carbon-nano sol treatments.