排脓散对结肠癌小鼠的抗肿瘤作用

Anti-tumor effects of Painong Powder on mice with colon cancer

目的考察排脓散对结肠癌小鼠的保护作用及炎症因子水平的影响。方法采用氧化偶氮甲烷(AOM)/葡聚糖硫酸钠(DSS)诱导结肠癌小鼠模型,并在诱导过程中给予排脓散(1.6 g生药/kg)干预。苏木精/伊红染色观察结肠组织病理形态,免疫组化染色检测结肠组织炎症因子白介素-4(IL-4)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)蛋白表达。结果与模型组比较,排脓散提取物可降低结肠癌小鼠肿瘤数目、增加结肠长度(P<0.01)。同时排脓散下调IL-6、TNF-α蛋白表达(P<0.01),上调IL-4、IFN-γ蛋白表达(P<0.01)。结论排脓散对AOM/DSS诱导结肠癌小鼠模型具有显著的抗肿瘤作用,可能与调节结肠部位炎症因子水平相关。

AIM To investigate the protective effects of Painong Powder on mice with colon cancer and its impact on inflammatory factor levels.METHODS During azoxymethane(AOM)/dextran sulphate sodium(DSS)induction of mouse model of colon cancer,simultaneous Painong Powder use was applied(1.6 g crude drug/kg).Hematoxylin/eosin staining was used to observe the pathological morphology of colon tissues.Immunohistochemical staining was used to detect the protein expressions and distribution of inflammatory factors IL-4,IL-6,TNF-αand IFN-γ.RESULTS The extract of Painong Powder decreased tumors number and increased the length of colon in mice with colon cancer(P<0.01).And down-regulated protein expressions of IL-6 and TNF-α(P<0.01),and up-regulated protein expressions of IL-4 and IFN-γwere observed(P<0.01).CONCLUSION Painong Powder has significant anti-tumor effect on AOM/DSS-induced colon cancer in mouse models,which may associate with the regulation of the levels of inflammatory factors in the colon.