血清miR-155联合IL-6检测对肝细胞肝癌预后的评估价值

The prognostic value of serum miR-155 combined with IL-6 in patients with HCC

目的研究血清microRNA-155(miR-155)联合白细胞介素-6(IL-6)水平检测对肝细胞肝癌(HCC)患者预后的评估价值。方法选取50例HCC患者,手术切除癌组织;另外选择同期50例体检健康者作为对照组。采用酶联免疫吸附试验(ELISA)检测及荧光定量PCR法检测两组IL-6及miR-155水平,比较两组miR-155、IL-6水平,ROC曲线分析IL-6联合miR-155水平与HCC患者预后的关系,比较其灵敏度、特异度及准确度,同时对HCC患者预后进行多元回归分析。结果HCC组IL-6、miR-155水平高于对照组(P<0.05)。IL-6=9.7μg/L,miR-155=2.5为截断点,IL-6联合miR-155测定HCC患者预后的灵敏度、特异度及准确度均高于IL-6或miR-155单独测定(P<0.05)。TNM分期、肿瘤直径、病理分级、淋巴转移、门静脉侵犯、高水平miR-155、高水平IL-6是HCC患者预后的危险因素(P<0.05)。肿瘤大小、淋巴转移、门静脉侵犯、高水平miR-155及高水平IL-6是影响HCC患者预后的独立危险因素(P<0.05)。结论IL-6和miR-155联合检测可作为HCC诊断的指标之一,同时也可作为评估HCC患者预后的独立预测因子。

Aim To evaluate the prognostic value of serum microRNA-155(miR-155)combined with interleukin-6(IL-6)in patients with HCC.Methods 50 patients with HCC were selected and the carcinoma tissue was removed by operation.Another 50 healthy patients were selected as the control group.The levels of IL-6 and miR-155 in the two groups were detected by enzyme-linked immunosorbent assay(ELISA)and fluorescence quantitative PCR.The levels of miR-155 and IL-6 in the two groups were compared.The relationship between IL-6 combined miR-155 and prognosis of HCC patients was analyzed by ROC curve,and the sensitivity,specificity and accuracy were compared.Results The levels of IL-6 and miR-155 in HCC group were higher than those in control group(P<0.05).IL-6=9.7μg/L and miR-155=2.5 were cut-off points.The sensitivity,specificity and accuracy of IL-6 combined with miR-155 were higher than those of IL-6 or miR-155 alone(P<0.05).TNM stage,tumor diameter,pathological grade,lymph node metastasis,portal vein invasion,high level of miR-155 and high level of IL-6 were risk factors for the prognosis of HCC patients(P<0.05).Tumor size,lymph node metastasis,portal vein invasion,high level of miR-155 and high level of IL-6 were independent risk factors for the prognosis of HCC patients(P<0.05).Conclusion The combined detection of IL-6 and miR-155 can be used as one of the indicators for the diagnosis of HCC,and as an independent predictor for the prognosis of HCC patients.