不同丛枝菌根真菌组合对滇重楼光合生理和化学成分的影响

Effect of Different Combinations of Arbuscular Mycorrhiza on Paris polyphylla var. yunnanensis and Chemical Components

目的:研究接种不同丛枝菌根(AM)真菌组合对滇重楼幼苗的生长发育和入药品质的影响,为培育优质滇重楼药材提供参考。方法:通过室温盆栽接种试验方法,在灭菌栽培土壤中分别接种9组不同AM真菌组合,以不接种为空白组,观察不同AM真菌组合对滇重楼幼苗的根系侵染率、光合参数、生理指标及化学成分等的影响。结果:接种外源AM真菌组合显著增加滇重楼根际土壤中的孢子密度、根系AM真菌的侵染率和侵染强度,以及根内琥珀酸脱氢酶和碱性磷酸酶活性;与空白组相比,不同AM真菌处理组对滇重楼叶片叶绿素含量、丙二醛、可溶性糖和可溶性蛋白含量、抗氧化酶活性等均有不同程度的提高;接种混合AM真菌可以诱导滇重楼次生代谢过程的变化,总皂苷含量在T1至T2时期呈上升趋势,T2至T4时期急速减少,到T5时期有所提高。结论:不同混合AM真菌能与滇重楼根系形成良好的共生关系,提高菌根生活力,增强滇重楼对外界不利环境的抗逆性,促进滇重楼的生长发育以及提高根茎品质。从综合接种效应来看S5,S8,S9组AM真菌组合效果最佳,为滇重楼菌根生物技术的运用提供了参考价值。

Objective:To study the effects of different arbuscular mycorrhizal(AM)fungi combinations on the growth and quality of Paris polyphylla var. yunnanensis seedlings,in order to provide reference for the cultivation of high quality P. polyphylla var. yunnanensis. Method: Through the room temperature pot inoculation test method,nine groups of different AM fungi combinations were inoculated into sterilized soil,and the control group was not inoculated. The effects of different AM fungi combinations on root infection rate,photosynthetic parameters,physiological indexes and chemical components of P. polyphylla var. yunnanensis seedlings were observed. Result: The spore density in rhizosphere soil,the infection rate and intensity of AM fungi in roots,and the activities of succinate dehydrogenase and alkaline phosphatase in roots of P. polyphylla var. yunnanensis were significantly increased by inoculating the combination of exogenous AM fungi. Compared with the control group,the contents of chlorophyll,malondialdehyde,soluble sugar,soluble protein content and the activities of antioxidant enzymes in leaves of P. polyphylla var. yunnanensis were increased in different AM fungi treatment groups. The content of total saponin increased from T1 to T2,decreased rapidly from T2 to T4,and increased at T5. Conclusion: Different mixed AM fungus can form a good symbiotic relationship with the roots of P. polyphylla var. yunnanensis,improve the mycorrhizal vitality,enhance the resistance of P. polyphylla var. yunnanensis to adverse environment,promote the growth and development of P. polyphylla var. yunnanensis and improve the quality of rhizome. According to the comprehensive inoculation effect,the combination of S5,S8 and S9 AM fungus has the best effect,which provides reference value for the application of mycorrhizal biotechnology of P. polyphylla var. yunnanensis.