ELA对骨髓间充质干细胞氧化应激损伤的保护作用

Protective Effect of ELA on Oxidative Stress Injuryof Bone Marrow Mesenchymal Stem Cells

目的:观察ELABELA(ELA)对过氧化氢(H_(2)O_(2))诱导骨髓间充质干细胞(Mesenchymal stem cells),ELA(500 nM,1μM,5μM,10μM)组。利用H_(2)O_(2)诱导MSCs凋亡模型,即H_(2)O_(2)组细胞加入含400μM H_(2)O_(2)的无血清培养基,在37℃条件下培养6小时。MTS法检测细胞增殖及细胞活力,DCFH-DA荧光探针法检测细胞内活性氧(ROS)含量。结果:与Control组相比,H_(2)O_(2)组细胞的存活能力显著降低(P<0.05),伴有细胞内活性氧水平明显升高(P<0.05);与H_(2)O_(2)组相比,ELA组细胞的存活率显著增加(P<0.05),且细胞内活性氧水平显著降低(P<0.05),其中以5μMELA治疗对MSCs的保护作用最佳。结论:ELA能够减轻过H_(2)O_(2)诱导的MSCs氧化应激损伤,其中以5μM浓度ELA的保护效应以达到最佳。

Objective:Toobserve the effect of ELABELA(ELA)on the injury of bone marrow mesenchymal stem cells(MSCs)induced by hydrogen peroxide(H_(2)O_(2))and investigate the optimum concentration.Methods:The MSCs cultured in vitro were divided into Control group,H_(2)O_(2) group,ELA(500 nM,1μM,5μM,10μM)groups.The apoptosismodel ofMSCswas induced by H_(2)O_(2),that is,the H_(2)O_(2) group cells were exposed to serum-free medium containing400μM H_(2)O_(2) and cultured at 37℃for 6 hours.MTS was used to detect cell proliferationandviability.DCFH-DA fluorescence probe method was detected to evaluate the level of intracellular reactive oxygen species(ROS).Results:Compared with the Control group,the cell viability of the H_(2)O_(2) group was remarkably reduced(P<0.05),accompanied by a significant increase in intracellularROSlevel(P<0.05).Compared with the H_(2)O_(2) group,the cell survival rate of ELA groupssignificantly increased(P<0.05),and the level of intracellular ROS was dramatically reduced(P<0.05).Among them,treatment with 5μM ELA had the best protective effect on MSCs.Conclusion:ELA can reduce the oxidative stress damage of MSCs induced by H_(2)O_(2),and the protective effect of ELA at a concentration of 5μM is optimal.