猪肝脏葡萄糖转运蛋白GLUT1和GLUT2的表达与定位

Expression and localization of glucose transporter GLUT1 and GLUT2 in pig liver

[目的]本文旨在探讨葡萄糖转运蛋白1(GLUT1)和葡萄糖转运蛋白2(GLUT2)在猪肝细胞的表达与定位。[方法]采用免疫组织化学技术、Western Blot和qRT-PCR技术检测了GLUT1和GLUT2在猪肝脏的表达和定位,并与大鼠肝脏对比。[结果]GLUT1、GLUT2蛋白和mRNA在猪肝脏中均有表达。免疫组织化学结果显示,GLUT1主要定位于猪肝细胞的细胞质,大鼠肝细胞定位于细胞质和细胞膜;GLUT2定位于猪肝细胞连接面和胆小管膜侧,大鼠肝细胞基底外侧膜。[结论]本研究发现葡萄糖转运蛋白GLUT1和GLUT2均表达定位于猪肝细胞,提示这2种葡萄糖转运蛋白可能参与猪肝细胞从血窦内摄取葡萄糖或肝细胞间葡萄糖转运的调节。

[Objective]This research was aimed to investigate the expression and localization of glucose transporter 1(GLUT1)and glucose transporter 2(GLUT2)in porcine hepatocytes.[Methods]The expression and localization of GLUT1 and GLUT2 in pig liver were tested by immunohistochemistry,Western Blot and qRT-PCR,and compared with those in rat liver.[Results]The results showed that GLUT1 and GLUT2 protein and mRNA were expressed in pig liver.Immunohistochemical results showed that GLUT1 was mainly located in the cytoplasm of porcine hepatocytes,the cytoplasm and cell membrane of rat hepatocytes.GLUT2 was located on the junction surface of porcine hepatocytes and the side of the bile duct membrane of pig hepatocytes,while on the basolateral membrane of hepatocytes near the blood sinus in rat liver.[Conclusion]This study found that both GLUT1 and GLUT2 were expressed and located in porcine hepatocytes,suggesting that these two glucose transporters may be involved in the uptake of glucose from blood by hepatocytes and the regulation of glucose transport between hepatocytes.