凝血因子Ⅶ及其重组表达新进展

Research Progress of Human Coagulation Factor Ⅶ and the Recombinant Expression Systems

凝血因子Ⅶ是一种维生素K依赖型的单链糖蛋白,在凝血过程中发挥着极其重要的作用,在临床上有广泛的应用,可用于伴有抑制物的血友病、先天性FⅦ缺乏症、血小板无力症及外科手术或严重外伤导致的创伤出血等止血用途。基因重组技术提供了能够大规模制备人凝血因子Ⅶ的有效途径,近年来已尝试并建立了多种人凝血因子Ⅶ的重组表达系统。对重组人凝血因子Ⅶ蛋白在酵母细胞、哺乳动物细胞、转基因动物这三类表达系统中的发展和应用概况进行了综述,介绍了不同表达系统的特点并比较了FⅦ在不同哺乳动物细胞系中蛋白质翻译后修饰的差别,为重组人凝血因子Ⅶ在重组表达系统上的进一步开发提供参考。

Human coagulation factorⅦis a single chain glycoprotein that is vitamin K-dependent and plays an extremely important role in the process of coagulation,which has been widely used in the clinic.It can be used for hemostatic purposes such as hemophilia with inhibitors,congenital FⅦdeficiency,thrombocytopenia and traumatic bleeding caused by surgery or severe trauma.The gene recombination technology is an attractive methed which provides more effective ways to produce recombinant human coagulation factorⅦon a large scale.In recent years,a variety of recombinant expression systems of human coagulation factorⅦhave been tried and established.In this paper,it is focused on the development and application of recombinant human coagulation factorⅦin the expression systems of yeasts,mammalian cells and transgenic animals and compares the characteristics of post-translational modification of different mammalian cells,so as to provide reference for the further development of recombinant human coagulation factorⅦin the recombinant expression system.