出芽短梗霉菌PA−2侵染对藜叶片代谢的影响

Effect of Aureobasidium pullulans PA−2 Metabolism of Chenopodium album Leaves

为探明出芽短梗霉菌Aureobasidium pullulans PA−2侵染对藜Chenopodium album叶片代谢影响,采用基于液相色谱−质谱联用技术(LC−MS)的代谢组学方法,对出芽短梗霉菌PA−2侵染藜叶片差异代谢产物进行分析。结果表明,通过正交偏最小二乘法判别分析(OPLS−DA),PA−2孢子处理藜叶片24 h后和处理前两组共筛选到185个具有显著差异(VIP>1,P<0.05)的代谢产物,其中有148个上调,37个下调,腺苷和甜菜碱可以作为24 h后的主要生物标志物。KEGG通路富集分析发现,这些差异代谢物主要富集在全局和概述地图、氨基酸代谢、碳水化合物代谢通路等3条代谢通路中。藜叶片中嘌呤代谢、次级代谢产物生物合成、氨基酸代谢和碳水化合物代谢变化可能是PA−2侵染藜的主要机制。本研究为进一步研究出芽短梗霉菌侵染藜的生防机制提供了理论基础。

To clarify the effect of Aureobasidium pullulans PA-2 infection on metabolism of Chenopodium album leaves,the metabolomics method based on liquid chromatography-mass spectrometry(LC-MS)was used to analyze the differential metabolites in C.album leaves infected by the strain PA-2.Through orthogonal partial least squares discriminant analysis(OPLS-DA),a total of 187 metabolites with significant differences(VIP>1 and P<0.05)were screened 24 h after the treatment of PA-2.Among them,148 were up-regulated and 37 were down-regulated.Adenosine and betaine can be used as the main biomarkers after 24 h.KEGG enrichment pathway analysis indicated that these differential metabolites were mainly enriched in 3 metabolic pathways,including global and overview maps,amino acid metabolism and carbohydrate metabolism.The obvious changes in purine metabolism,biosynthesis of secondary metabolites,amino acid metabolism and carbohydrate metabolism in C.album leaves may participate in the PA-2 infection to C.album.The above results provide a theoretical basis for further research on the biocontrol mechanism of PA-2 infecting C.album.