大黄酸对胰腺癌细胞增殖和迁移的影响及机制研究

Effect and Mechanism of Rhein on Proliferation and Migration of Human Pancreatic Cancer Cells

目的:研究大黄酸对人胰腺癌细胞增殖和迁移的影响,并探讨其作用机制。方法:用不同浓度的大黄酸处理人胰腺癌MiaPaCa-2细胞,CCK8法检测大黄酸对MiaPaCa-2细胞增殖的影响;于常氧和缺氧条件下培养MiaPaCa-2细胞,Transwell法检测大黄酸对胰腺癌细胞迁移的影响,并用Western blot法检测细胞缺氧诱导因子-1α(HIF-1α)、上皮型钙黏附蛋白(E-cadherin)与锌指转录因子(Snail)的表达。结果:CCK8结果显示,大黄酸可以抑制MiaPaCa-2细胞的增殖,其抑制作用具有剂量依赖性和时间依赖性(P<0.05)。Transwell迁移实验表明,大黄酸对常氧和缺氧条件下的MiaPaCa-2细胞迁移均有抑制作用(P<0.05)。与对照组相比,大黄酸能抑制HIF-1α和Snail的表达,且抑制作用具有剂量依赖性,大黄酸能促进E-cadherin表达升高(P<0.05)。结论:大黄酸抑制人胰腺癌细胞系MiaPaCa-2的增殖和迁移,其抑制作用可能与抑制MiaPaCa-2细胞中HIF-1α的表达有关。

Objective To study the effect and mechanism of rhein on proliferation and migration of human pancreatic cancer cells.Methods Human pancreatic cancer MiaPaCa-2 cells were treated with different concentrations of rhein and the proliferation of MiaPaCa-2 cells was assessed by CCK8 method.MiaPaCa-2 cells were cultured in normoxic and hypoxic conditions and cells migration was examined by transwell.Hypoxia inducible factor-1α(HIF-1α),E-cadherin and Zinc Finger Transcription Factor(Snail)were determined by western blot assay.Results The results of CCK8 assay showed that rhein inhibited the proliferation of pancreatic cancer MiaPaCa-2 cells in a dose-and time-dependent manner(P<0.05).Rhein also inhibited the migration of MiaPaCa-2 cells in both normoxic and hypoxia conditions(P<0.05).Compared with the control group,rhein inhibited the expression of HIF-1αand Snail in adose-dependent manner and rhein could increase the expression of E-cadherin(P<0.05).Conclusion Rhein can inhibit the proliferation and migration of human pancreatic cancer cells,which may be related to the inhibition of HIF-1αexpression in MiaPaCa-2 cells.