银杏叶提取物对青春期乏运动大鼠行为学的影响

Effect of ginkgo biloba extract on behaviors in rat with adolescent sedentariness

目的探讨银杏叶提取物对青春期乏运动大鼠行为学的影响及其可能机制。方法将24只4周龄SD雄鼠随机分为正常对照组(NC)、乏运动组(AS)以及银杏叶提取物处理组(GBE),干预性饲养至8周龄,进行旷场实验及高架十字迷宫实验检测行为学改变。比色法检测脑组织匀浆丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。Western blotting检测脑实质糖原合成激酶3β(GSK-3β)和β-连环蛋白(β-catenin)蛋白表达水平。结果旷场实验显示,AS组大鼠自主活动增加,而在旷场中央区域停留时间及次数减少;高架十字迷宫实验显示,AS组大鼠进入开臂时间及次数均减少。GBE干预有效逆转AS诱导的焦虑样行为(P<0.05)。与NC组相比,AS组GSK-3β和β-catenin比值上调,MDA含量增加,SOD活性降低;而GBE组GSK-3β和β-catenin比值明显下调,MDA含量下降,SOD活性增加(P<0.05)。结论银杏叶提取物对青春期乏运动大鼠焦虑样行为具有改善作用,Wnt/β-catenin信号通路及抗氧化调节可能参与脑保护作用。

Objective To investigate the effect of Ginkgo biloba extract on behaviors in rat with adolescent sedentariness and its possible mechanism. Methods Twenty-four male SD rats (4-week-old) were randomly divided into normal control (NC),adolescent sedentarines (AS),and Ginkgo biloba extract (GBE) groups. After 4 weeks intervention with GBE,open-field test and elevated plus-maze test were performed to detect the behavioral changes. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in brain were determined by colorimetric method.Protein levels of glycogen synthase kinase 3β (GSK-3β) and β-catenin in cerebral white matter were determined by Western blotting. Results In open-field test,it was shown that the autonomic activity of rats in AS group increased,while the central regional travel time was reduced. Duration and number of residence in the open arm of elevated plus-maze test decreased in AS group. These anxiety-like behaviors were ameliorated by GBE intervention. Compared with the NC group,GSK-3β/β-catenin ratio and the content of MDA was upregulated in AS group while downregulated in GBE group. And activity of SOD were downregulated in AS group while significantly upregulated in GBE group (P< 0. 05). Conclusion Ginkgo biloba extract ameliorate anxiety-like behavior in rat with adolescent sedentariness. Wnt/β-catenin pathway and antioxidant regulation may play a cerebral protective role.