沉默UHMK1对乳腺癌细胞增殖、迁移和侵袭的影响

Effects of silencing UHMK1 on proliferation,migration and invasion in breast cancer cells

目的探讨干扰乳腺癌细胞系中UHMK1的表达对乳腺癌细胞体外增殖、迁移和侵袭能力的影响。方法应用短发夹RNA(short hairpin RNA,shRNA)干扰乳腺癌细胞株MDA-MB-231中UHMK1的表达,分别采用qRT-PCR和Western blot实验检测shRNA的干扰效率。应用MTT实验检测细胞活力;克隆形成实验检测细胞克隆形成能力;Transwell实验检测细胞迁移及侵袭能力。结果UHMK1-shRNA可显著干扰乳腺癌细胞MDA-MB-231中UHMK1的表达(P<0.01)。与对照组Plko.1相比,干扰UHMK1表达可使乳腺癌细胞MDA-MB-231的增殖、迁移及侵袭能力显著下调(P<0.01)。结论UHMK1表达可能参与乳腺癌生物学行为的调控。

Purpose To study the effect of silencing UHMK1 expression of breast cancer cells on the proliferation,migration and invasion in vitro.Methods Short hairpin(shRNA)was used to interfere with the expression of UHMK1 in breast cancer cell line MDA-MB-231.qRT-PCR and Western blot were used to detect the interference efficiency of shRNA.MTT assay was used to detect the effect on cell viability.The change of cell proliferation ability was detected by clone formation assay.Transwell assay detected effect on cell migration and invasion.Results UHMK1-shRNA significantly interfered with UHMK1 expression in breast cancer cells MDA-MB-231(P<0.01).Compared with the control group Plko.1,the interference of UHMK1 expression significantly downregulated the capacity of proliferation,migration and invasion in breast cancer cell line MDA-MB-231(P<0.01).Conclusion UHMK1 expression may be involved in the regulation of biological behavior of breast cancer.