原花青素对多囊卵巢综合征大鼠卵巢及PI3K/Akt信号通路的影响

Effects of procyanidins on ovary and PI3K/Akt signaling pathway in PCOS rats

目的探讨不同浓度原花青素对多囊卵巢综合征(PCOS)大鼠的影响和可能的作用机制。方法将50只大鼠按照平均体重分为A组(未建立模型)、B组(PCOS大鼠)、C组(B组+50 mg/kg原花青素)、D组(B组+100 mg/kg原花青素)和E组(B组+150 mg/kg原花青素),每组10只。ELISA血清检测雌激素含量,免疫组化检测子宫容受性指标LIF及整合素β3,HE染色检测子宫组织病理,免疫印迹及PCR检测PI3K/Akt蛋白及m RNA表达。结果与A组比较,B组大鼠卵巢体积、相对质量、LH均升高(P<0.05),FSH、E2、P水平、LIF、整合素β3蛋白、p-PI3K、p-Akt蛋白及PI3K、Aktm RNA均降低(P<0.05);与B组比较,C~E组大鼠卵巢体积、相对质量、LH均降低(P<0.05),FSH、E2、P水平、LIF、整合素β3蛋白、p-PI3K、p-Akt蛋白及PI3K、Akt mRNA均升高(P<0.05);E组与C组相比,大鼠卵巢体积、相对质量、血清雌激素水平、LIF和整合素β3蛋白均存在明显差异(P<0.05);C~E组大鼠子宫组织中p-PI3K、p-Akt蛋白及PI3K、Akt mRNA任意两两比较均存在统计学差异(P<0.05)。结论原花青素能够上调PCOS大鼠血清中FSH、E2及P水平,增加子宫容受性指标LIF和整合素β3表达,改善雌激素紊乱,增加子宫容受性,呈现浓度依赖性改善病情,其研究机制可能与促进PI3K/Akt表达相关。

Objective To explore the mechanism of action of different concentrations of proanthocyanidins on polycystic ovary syndrome(PCOS) rats based on PI3 K/Akt signaling pathway. Methods 50 rats were divided into group A(no model established), group B(PCOS rats), group C(group B + 50 mg/kg proanthocyanidin), and group D(group B + 100 mg/kg proanthocyanidin) according to their average body weight. And E group(B group + 150 mg/kg proanthocyanidin), each group has 10 animals. ELISA serum test estrogen content, immunohistochemistry test uterine receptivity index LIF and integrin β3, HE staining test uterine tissue pathology, Western blot and PCR test PI3 K/Akt protein and mRNA expression. Results Compared with group A, the ovarian volume, relative mass and LH of group B increased(P<0.05), FSH, E2, P levels, LIF, integrin β3 protein, p-PI3 K, p-Akt protein and Both PI3 K and AktmRNA were reduced(P<0.05);compared with group B, the ovarian volume, relative mass, and LH of rats in groups C–E were reduced(P<0.05), and the levels of FSH, E2, P, LIF, and integrin β3 protein, P-PI3 K, p-Akt protein, PI3 K, AktmRNA were all increased(P<0.05);compared with group C, rat ovarian volume, relative mass, serum estrogen level, LIF and integrin β3 protein were all There are significant differences(P<0.05);there are statistical differences in any pair of p-PI3 K, p-Akt protein, PI3 K, and Akt mRNA in the uterine tissue of rats in the C–E group(P<0.05). Conclusion Proanthocyanidins can up-regulate the serum FSH, E2 and P levels in PCOS rats, increase the expression of uterine receptivity indicators LIF and integrinβ3, improve estrogen disorders, increase uterine receptivity, and present a concentration-dependent improvement of the disease. Its research The mechanism may be related to the promotion of PI3 K/Akt expression.