重度脑外伤患者血清miR-146a水平与继发性癫痫的相关性分析

Correlation between serum miR-146a level and secondary epilepsy in patients with severe brain injury

目的探讨重度脑外伤患者血清微小RNA-146a(miR-146a)水平与继发性癫痫的相关性。方法选取2018年1月至2020年2月该院重度脑外伤住院患者104例为研究对象,按照是否继发癫痫分为重度脑外伤继发性癫痫组61例,单一重度脑外伤组43例,同期选择该院体检健康者70例为对照组。采用酶联免疫吸附试验(ELISA)法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)水平,采用实时荧光定量PCR(qRT-PCR)测定miR-146a水平,Pearson相关分析miR-146a与TNF-α、IL-6、IL-1β相关性,对影响继发性癫痫的相关因素进行Logistic回归分析。结果与对照组相比,重度脑外伤继发性癫痫组、单一重度脑外伤组患者血清miR-146a、TNF-α、IL-6、IL-1β水平较高(P<0.05),与单一重度脑外伤组相比,重度脑外伤继发性癫痫组患者血清miR-146a、TNF-α、IL-6、IL-1β水平较高(P<0.05)。Pearson相关分析显示重度脑外伤继发性癫痫患者血清miR-146a与TNF-α、IL-6、IL-1β水平呈正相关(r=0.580、0.505、0.474,P<0.05)。多因素Logistic回归分析显示miR-146a、TNF-α、IL-6、IL-1β水平升高是重度脑外伤继发性癫痫的危险因素。结论重度脑外伤继发性癫痫患者血清miR-146a水平上调,检测血清miR-146a水平有助于诊断重度脑外伤继发性癫痫的发生。

Objective To explore the correlation between serum miR-146a level and secondary epilepsy in patients with severe brain injury.Methods A total of 104 inpatients with severe brain trauma were selected from January 2018 to February 2020,according to whether or not secondary epilepsy occurred,they were divided into severe brain trauma with secondary epilepsy group(n=61)and simplex severe brain trauma group(n=43).At the same time,70 cases of healthy people in the same period were selected as control group.The levels of serum tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)were detected by enzyme linked immunosorbent assay(ELISA),and the level of miR-146a was measured by real-time fluorescence quantitative PCR(qRT-PCR).Pearson analysis was used to analyze the correlations between miR-146a and TNF-α,IL-6,IL-1β.Logistic regression analysis was used to analyze the related factors affecting secondary epilepsy.Results Compared with the control group,the levels of serum miR-146a,TNF-α,IL-6 and IL-1βwere significantly higher in the severe brain trauma with secondary epilepsy group and the simplex severe brain trauma group(P<0.05).Compared with the simplex severe brain trauma group,the levels of serum miR-146a,TNF-α,IL-6 and IL-1βin the severe brain trauma with secondary epilepsy group were significantly higher(P<0.05).Pearson correlation analysis showed that serum miR-146a was positively correlated with TNF-α,IL-6 and IL-1βlevels in patients with severe brain trauma with secondary epilepsy(r=0.580,0.505,0.474,P<0.05).Multivariate Logistic regression analysis showed that high levels of serum miR-146a,TNF-α,IL-6 and IL-1βwere risk factors for severe brain trauma with secondary epilepsy.Conclusion The serum miR-146a level is up-regulated in severe brain trauma with secondary epilepsy.Detection of serum miR-146a level is helpful to prevent severe brain trauma with secondary epilepsy.