CARM1、p16INK4a在子宫颈鳞状细胞癌中的表达及临床意义

The expression and clinical significance of CARM1 and p16INK4a in cervical squamous cell carcinoma

目的检测子宫颈鳞状细胞癌(CSCC)、宫颈上皮内病变及正常宫颈组织中组蛋白精氨酸甲基转移酶1(CARM1)、p16INK4a的表达,探讨两者在子宫颈鳞癌发生发展中的作用。方法运用免疫组化技术检测CSCC、宫颈上皮内病变及正常宫颈组织中CARM1、p16INK4a表达,分析二者表达与子宫颈鳞状细胞癌临床病理特征及生存预后的关系,并评价两者的相关性。结果CARM1、p16INK4a蛋白阳性表达率在正常子宫颈组织、低级别鳞状上皮内病变(LSIL)、高级别鳞状上皮内病变(HSIL)、CSCC中呈递增趋势;子宫颈鳞状细胞癌组织CARM1蛋白阳性表达率为70.24%,与组织分化程度和浸润深度相关;p16INK4a蛋白阳性表达率为92.86%,表达与组织分化程度相关;单因素分析显示CARM1表达与OS相关,多因素分析显示仅FIGO分期是OS独立预后因素;CARM1和p16INK4a表达相关系数Rs=0.325,呈弱正相关关系。结论CARM1、p16INK4a可能参与了子宫颈鳞癌发生发展进程,是子宫颈鳞状细胞癌的不良预后因子,两者可能存在部分协同作用。

Objective To detect the expression of CARM1 and p16INK4a proteins in cervical squamous intraepithelial lesions and cervical squamous cell carcinoma(CSCC)tissues,and discuss their action and interaction during the occurrence and development of CSCC.Methods Immunohistochemistry methods were used to detect the expression of CARM1 and p16INK4a proteins in cervical intraepithelial lesions and CSCC tissues,the relationship among their expression,clinicopathological characteristics and survival prognosis of CSCC were analyzed.Results The expression of CARM1 and p16INK4a coincidently increased in normal cervical tissue,low-grade cervical squamous intraepithelial lesions(LSIL),high-grade squamous intraepithelial lesions(HSIL)and CSCC tissues.The positive expression rate of CARM1 in CSCC tissues was 70.24%,which was correlated with differentiation and invasive depth of CSCC.The positive expression rate of P16INK4a in CSCC tissues was 92.86%,which was related to differentiation of CSCC.Kaplan-Meiri analysis revealed that CARM1 expression was correlated with overall survival(OS).Cox proportional hazards model showed that stage was the only independent prognostic factor of CSCC.The correlation coefficient of CARM1 and p16INK4a expression was 0.325 by Spearman correlation analysis.Conclusions CARM1 and p16INK4a proteins might be involved in the occurrence and development of CSCC,and also were adverse prognostic factors.There may be some synergy between them.