鹅细小病毒辽宁分离株全基因测序与遗传演化分析

Whole gene sequencing and genetic evolution analysis of Liaoning isolates of goose parvovirus

为研究辽宁地区鹅细小病毒的遗传演化及分子生物学等特征,参考GenBank已公布的鹅细小病毒DY16毒株设计8对引物,对辽宁分离株全基因分段进行扩增并测序,采用DNAMEN软件将测序结果依次拼接后进行序列比对分析。结果表明:辽宁分离株基因序列全长5 106 bp,并命名为LNGPV20。基因组5’端和3’端具有一致的末端倒置重复序列(ITR),均由444个碱基组成,包括362个碱基配对形成的回文茎部、43个碱基构成的泡区以及39个碱基不参与序列的反向互补配对。辽宁分离毒株与14个毒株全基因序列同源性在93.2%~98.9%之间,表明国内鹅细小病毒全基因序列同源性较高,可能是近年来养鹅业迅速发展,鹅苗流通增加,交叉感染所致。

In order to study the characteristics of genetic evolution and molecular biology of goose parvovirus in Liaoning area, eight pairs of primers were designed with reference to the goose parvovirus DY16 strain published in GenBank, and the whole gene segment of the Liaoning isolate was amplified and sequenced, using DNAMEN software. The sequencing results are sequentially assembled and then sequenced and analyzed. The results showed that the gene sequence of the Liaoning isolate was 5 106 bp in length and was named LNGPV20. The 5’end and 3’end of the genome have identical terminal inverted repeats(ITR), both of which consist of 444 bases, including a palindrome formed by 362 base pairings, a 43-base bubble region, and 39 Each base does not participate in the reverse complementary pairing of the sequence. The whole gene sequence homology between the isolated strains in Liaoning and 14 strains was between 93.2% and 98.9%, indicating that the whole gene sequence of the domestic goose parvovirus has high homology, which may be the result of the increasing seedling circulation caused by the cross-infection as the rapid development of the goose industry in recent years.