灵芝液体浅层静置培养高效生产三萜的研究

An efficient strategy to enhance triterpenoid production by liquid superficial-static culture(LSSC)of Ganoderma lingzhi

三萜是灵芝Ganoderma lingzhi中重要的活性物质。本研究采用液体浅层静置培养方式(LSSC)提高灵芝三萜的产量。结果表明,在T25细胞培养瓶中的最佳培养条件为初始培养体积2mL,接种量7.0g(菌体湿重/L),在48h补加2mL培养液,发酵7d,三萜产量可达(32.95±0.51)mg/g,为摇瓶培养最高产量的2.09倍。进一步经不锈钢平盘放大研究发现,三萜最高产量为(65.91±0.84)mg/g,为摇瓶培养的4.19倍。形态学研究表明,浅层静置培养过程中形成的气生菌丝是提高三萜产量的关键因素。与深层液体发酵相比,液体浅层静置培养具有生长快,三萜产量高,发酵过程不需要搅拌等优点,具有良好的工业化应用前景。

Triterpenoid was recognized as the major bioactive molecule of Ganoderma lingzhi.In this work,liquid superficial-static culture(LSSC)process was used to enhance the production of triterpenoid.After optimizing,the best condition to produce triterpenoid in LSSC was initial medium volume 2 mL,inoculum size 7.0 g/L,adding medium 2 mL in 48 h and fermentative duration 7 d.Under the optimal conditions,the triterpenoid production scaled(65.91±0.84)mg/g,being 4.19-fold that in submerged culture.The results demonstrated that aerial mycelial formation was a key factor for triterpenoid biosynthesis of G.lingzhi.Compared with submerged culture,LSSC exhibited growing fast and high triterpenoid production and productivity needless to stir or shake,and had potential for industrial application.