RHBDF1在ccRCC中的临床意义及其免疫浸润情况

The clinical significance of RHBDF1 and its immune infiltration in ccRCC

[目的]探讨RNBDH1在肾透明细胞癌(ccRCC)中的临床意义及RHBDF1在ccRCC中免疫浸润的情况。[方法]从TCGA数据库下载ccRCC病人的mRNA表达及相关临床信息。然后提取出ccRCC病人的RHBDF1的单基因矩阵。评估了肿瘤与非肿瘤组织中的RHBDF1的差异表达。Kaplan-Meier生存分析RHBDF1的表达量与总体生存率(OS)之间的关系。同时运用Cox回归模型分析了各种临床信息与生存时间的关系。基因富集分析(GSEA)探究RHBDF1在ccRCC中的潜在作用。最后还探究了RHBDF1与免疫浸润的关系。[结果]RHBDF1在肿瘤组织中的表达量明显增高(P <0.01),并且RHBDF1的高表达与较差的OS相关(P <0.05)。Cox回归分析提示RHBDF1可以作为ccRCC患者的独立预后因素(P <0.05)。RHBDF1的高表达可能主要影响调节性T细胞和M2型巨噬细胞的含量,从而影响ccRCC的免疫微环境。经GSEA发现,RHBDF1在7条KEGG通路中显著富集(FDR q-value <0.05、NES> 1、Nom pvalue <0.05)。[结论]RHBDF1是判断ccRCC预后的生物学标记物,并可能通过Notch通路促进肿瘤的发生、发展,并能影响ccRCC的免疫浸润。

[Objective] To investigate the clinical significance of the human rhomboid family-1 gene( RHBDF1) in Clear Cell Renal Cell Carcinoma( ccRCC). And analysize the function of RHBDF1 in the immune infiltration in ccRCC. [Method]We download the transcriptome profiling data and clinical information of ccRCC from TCGA. Subsequently,we extracted the single-gene sequence matrix of RHBDF1 in ccRCC. And,we evaluated the differential expression of RHBDF1 between tumor and non-tumor tissue in ccRCC patients. Univariable and Multivariate Cox analysis were applied to analyze the influence of clinical factors on overall survival( OS). And,Kaplan – Meier curve was plot to show the correlation between differential expression of RHBDF1 and OS. In addition,we accessed the tumor microenvironment of ccRCC and relation between RHBDF1 and tumor immune infiltrates of ccRCC. Gene set enrichment analysis( GSEA) was used to investigate the potential role of RHDBF1 in ccRCC.[Result] The level of RHBDF1 Expression was differential significant in ccRCC patients than in normal ones. Higher RHBDF1 level would lead to worse OS( P < 0. 05) in ccRCC patients. Cox analysis indicated that RHBDF1 and age were independent risk factors( P < 0. 05) for ccRCC patients. The high expression of RHBDF1 in ccRCC mainly affected the proliferation level of T cells regulatory( Tregs) and Macrophages M2,thus affecting the immune infiltration level in ccRCC. GSEA identified seven pathways which were significantly enriched in Kyoto Encyclopedia of Genes and Genomes( KEGG)( FDR q-value <0. 05,NES > 1,Nom p-value < 0. 05).[Conclusion]RHBDF1 could be considered as a novel prognostic biomarker and might promote the progression of ccRCC by notch pathway. And RHBDF1 could influence the tumor immune infiltration in ccRCC.