十二烷基羟丙基磺基甜菜碱对表皮葡萄球菌生物被膜的影响

The effect of dodecyl hydroxypropyl sulfobetaine on biofilm of Staphylococcus epidermidis

通过表面活性剂十二烷基羟丙基磺基甜菜碱(Dodecyl hydroxypropyl sulfobetaine,DSB)对表皮葡萄球菌生物被膜内细菌代谢、生物被膜的清除效率和多糖胞间黏附素(Polysaccharide intercellular adhesion,PIA)形成等作用指标的检测,为DSB防止表皮葡萄球菌生物被膜污染提供理论及实践依据。以万古霉素为阳性对照,利用XTT减低法,评价DSB对表皮葡萄球菌生物被膜内细菌代谢的影响及对已形成的生物被膜的清除效率。采用刚果红培养基,观察DSB对PIA产生的影响,用激光共聚焦显微镜观察DSB对生物被膜作用的效果。结果显示,质量浓度为1024、512、256和128 mg/L的DSB对ATCC 35984生物被膜内细菌的代谢有极显著影响(P<0.001);质量浓度依次为64、32、16和8 mg/L的DSB在6、12和24 h对表皮葡萄球菌生物被膜均有显著的清除作用(P<0.001);质量浓度为16和8mg/L的DSB对PIA形成无明显影响。结果表明,DSB在影响表皮葡萄球菌生物被膜内细菌代谢及清除已形成的生物被膜等环节均可发挥显著作用。

To analyse the effect of dodecyl hydroxypropyl sulfobetaine(DSB)on bacterial metabolism,clearance efficiency and formation of polysaccharide intercellular adhesion of Staphylococcus epidermidis biofilm and to provide reliable theory and practical evidence for DSB to prevent biofilm contamination of S.epidermidis,vancomycin was used as positive control,XTT reduction was used to evaluate the clearance and metabolism of DSB on S.epidermidis biofilm,Congo red medium and laser scanning confocal microscope(LSCM)were used to observe the effect of DSB on PIA and biofilm,respectively.The results showed that 1024,512,256 and 128 mg/L of DBS had significantly effect on metabolism of ATCC 35984,and 64,32,16 and 8 mg/L of DBS had significantly effect on clearance in the 6,12 and 24 hours post treatment(P<0.001).However,16 and 8 mg/L of DSB had no significant effect on PIA formation.These results indicated that DSB significantly affects bacterial metabolism in biofilms of S.epidermidis and in scavenging formed biofilm.