棉花植株发病程度与土壤大丽轮枝菌含量的相关性

Study on correlation between Verticillium dahliae contents in soil and the disease degree of cotton individual plants

采用接种等量大丽轮枝菌的盆栽棉花,结合枯草芽孢杆菌处理和病圃地为正负对照,利用特异引物qPCR分析土壤中大丽轮枝菌含量,并用水洗涂布菌落计数来补充验证土壤中病原菌的含量。结果表明盆栽土壤菌含量在不同时间点之间存在显著差异;同一时间点不同盆栽土壤含菌量的变化也呈现明显差异,尤其是后期的时间点差异尤为显著;同时盆栽苗的发病程度也存在显著差异;协方差分析发现盆栽棉花单株的发病程度与土壤里大丽轮枝菌含量呈现显著的正相关关系;枯草芽孢杆菌处理和病圃地土壤大丽轮枝菌含量的分析也证实了这种相关性。结果为棉花黄萎病防治提供理论指导。

Asingle cotton plant grown in a pot was inoculated with the same amount of Verticillium dahliae combining with treatment with Bacillus subtilis and soil sample of disease nursery to explore the relationship between the V.dahliae content in the potted soil at different time points and the disease degree of the individual plants.The organism DNA in the soil was extracted,and V.dahliae content in soil was analyzed by qPCR with specific primers.The pathogens content in soil was further verified by colony counting of washing solution from the potted soil.The results showed that significant differences in V.dahliae content in the potted soil inoculated with equal amount of fungi at different time points were shown.And at the same time point,there was a significant difference in the change of the fungi content in the different potted soils,especially in the later stage of time points.Then covariance analysis showed that there was a significant positive correlation between the disease degree of a single potted plant and the V.dahliae content in the soil.The treatment of B.subtilis and the analysis of V.dahliae content in the disease nursery soil further confirmed that the disease degree of a single plant was positively related to the fungi content in the soil.In general,the results provide theoretical guidance for the control of cotton Verticillium wilt.