CARD-FISH技术在根系微生物中的研究进展

Advances of CARD-FISH technology in root-associated microorganisms

酶联荧光原位杂交技术(Catalyzed reporter deposition fluorescent in situ hybridization,CARD-FISH)是原位识别、追踪、可视化环境微生物的先进技术。它能在植物组织中精确定位目标微生物,帮助监测微生物的存在及其在根系的定植策略。随着分子微生物生态技术的进步,CARD-FISH与其他技术联用更能高效解析复杂生境中微生物的功能特性,如CARD-FISH与纳米二次离子质谱技术(nanometer secondary ion mass spectrometry,nanoSIMS)联用能在单细胞水平上同时表征微生物的代谢功能与系统发育特性。综述CARD-FISH的原理、步骤、发展及其联用技术,为CARD-FISH用于植物组织微生物的定位和功能分析提供依据和参考。

Catalyzed reporter deposition fluorescent in situ hybridization(CARD-FISH)is an advanced technology to identify,track,and visualize root-associated microorganisms.It can accurately locate specific microbes in plant tissues,help us monitor the presence of microorganisms and their colonization strategy in the root system,and it is a powerful tool for studying root-associated microorganisms.With the advancement of molecular microbial ecological techniques,the combination of CARD-FISH and other approaches can efficiently analyze the functional properties of microorganisms in complex habitats.For example,the combination of CARD-FISH and nanometer secondary ion mass spectrometry(nanoSIMS)can simultaneously characterize the metabolic function and the phylogenetic properties of microorganism at the single-cell level.This article reviewed the principles,steps,development and combined technique of CARD-FISH,in order to provide a basis and reference for CARD-FISH to be used in the localization and functional analysis of plant tissue microorganisms.