载脂蛋白A1对小鼠淋巴细胞频率及活性的影响

Effects of ApoA-Ⅰ on the frequencies and activities of mouse lymphocytes

以载脂蛋白A1 (ApoA-Ⅰ)基因敲除及转基因小鼠为基础,利用流式细胞术检测小鼠体内T细胞、B细胞和NK细胞及其亚型频率、表面活化型分子表达情况,探讨ApoA-Ⅰ水平对小鼠淋巴细胞频率及活性的影响。结果表明:相对于野生小鼠,生理状态下ApoA-Ⅰ基因敲除小鼠脾脏、外周血CD8^(+)T细胞及效应型(CD8^(+)NKG2D^(+)、CD8^(+)CD44^(+)CD62L^(-)、CD8^(+)CD44^(+)KLGR1^(+)、CD8^(+)IFN-γ^(+))细胞频率显著下调,而以上指标在ApoA-Ⅰ转基因小鼠中上调。CD4^(+)T细胞、调节性T细胞(CD4^(+)CD25^(+)FoxP3^(+))、B细胞和NK细胞频率在3组小鼠中均无显著差异。该研究说明ApoA-Ⅰ参与调控CD8^(+)T细胞活性,但对CD4^(+)T细胞、B细胞及NK细胞无显著影响。

The frequencies and surfaces markers of T cells, B cells and NK cells from ApoA-Ⅰ knockout and transgenic mice were detected to investigate the effects of apolipoprotein A1(ApoA-Ⅰ) levels on the frequencies and activities of mouse lymphocytes. Under physiological conditions, frequencies of CD8^(+)T cells and effector T cells(CD8^(+)NKG2 D^(+), CD8^(+)CD44^(+)CD62 L^(-), CD8^(+)CD44^(+)KLGR1^(+), CD8^(+)IFN-γ^(+)) were significantly down-regulated in the spleen and peripheral blood of ApoA-Ⅰ knockout mice compared with wild type mice, while the above cells were up-regulated in ApoA-Ⅰ transgenic mice. There was no significant difference in the frequency of CD4^(+)T cells, regulatory T cells(CD4^(+)CD25^(+)FoxP3^(+)), B cells, or NK cells in the three groups. Our study indicated that ApoA-Ⅰ was involved in the regulation of CD8^(+)T cell activity, but had no significant effect on CD4^(+)T cells, B cells and NK cells.