基于网络药理学的蓝芩口服液治疗新型冠状病毒肺炎的分子机制研究

Molecular mechanism of Lanqin oral solution in the treatment of COVID-19 based on network pharmacology

目的研究蓝芩口服液治疗新型冠状病毒肺炎的相关分子靶点及分子作用机制。方法使用TCMSP数据库检索蓝芩口服液的主要活性成分及其作用靶点。使用GeneCards数据库,检索新型冠状病毒肺炎的疾病相关基因靶点。使用韦恩图对蓝芩口服液主要活性成分作用靶点及新型冠状病毒肺炎相关基因靶点取交集得到共同基因靶点。使用Cytoscape3.7.2软件构建药物-活性成分-作用靶点网络图。使用STRING数据库构建共同作用靶点的蛋白质相互作用PPI网络。使用DAVID数据对共同基因靶点进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)通路富集分析。结果筛选出蓝芩口服液主要活性成分91种,作用基因靶点205个,其中43个为蓝芩口服液与新型冠状病毒肺炎的共同基因靶点,靶点包括丝裂原活化蛋白激酶8(MAPK8)、白介素6(IL6)、白介素10(IL10)、CC型修饰趋化因子配体2(CCL2)等。GO和KEGG富集分析共得到47个GO富集条目和59个KEGG富集条目,涉及癌症通路、HIF-1信号通路、MAPK信号通路等。结论蓝芩口服液在治疗COVID-19中有着多靶点调控、多方面作用的特点。

Objective To investigate the molecular targets and molecular mechanism of Lanqin oral solution in the treatment of coronavirus disease 2019(COVID-19)based on network pharmacology.Methods Traditional Chinese Medicine Systems Pharmacology(TCMSP)database was used to screen out the main active components of Lanqin oral solution and the targets of drug components.GeneCards database was used to screen out the gene targets which related to COVID-19.The common gene targets were obtained by the intersection of the main active components of Lanqin oral solution and the COVID-19-related gene targets using the Veen diagram.STRING database was used to construct a protein-protein interaction(PPI)network of common gene targets.Cytoscape 3.7.2 software was used to construct the drug-active-component-target network.DAVID database was used to construct the GO enrichment analysis and KEGG enrichment analysis.Results Lanqin oral solution has 91 main active components,and 205 gene targets were identified,of which 43 were the common gene targets of Lanqin oral solution and COVID-19,involving mitogen-activated protein kinase 8(MAPK8),interleukin 6(IL6),interleukin 10(IL10),CC chemokine ligand 2(CCL2),etc.The GO and KEGG enrichment analysis showed that there were 47 GO items and 59 KEGG items,involving pathways in cancer,HIF-1 signaling pathway and MAPK signaling pathway.Conclusion Lanqin oral solution has the characteristics of multi-target regulation and multi-function in the treatment of COVID-19.