黄瓜籽复合物对去势大鼠骨质疏松症的影响

Effects of Cucumber Seed Compound on Osteoporosis in Ovariectomized Rats

目的研究黄瓜籽复合物对骨质疏松症大鼠的防治作用。方法将24只雌性大鼠随机分为假手术组(Sham组)、模型组(OVX组)、雌二醇(E2)组(EV组)、黄瓜籽复合物组(QCL组)。双侧卵巢切除术建立骨质疏松症模型。术后灌胃给药12周后,用全自动生化仪测血清钙(S-Ca)、血清磷(S-P)水平;ELISA法检测E2、碱性磷酸酶(ALP)、I型胶原氨基端前肽(PINP)、I型胶原羧基端肽(CTX-I)、尿脱氧吡啶啉(DPD)水平;Micro-CT分析骨密度;HE染色观察股骨组织形态学改变。结果与Sham组相比,OVX组SCa、S-P、E2、PINP及骨密度水平降低(P<0.01),ALP、CTX-I、DPD水平升高(P<0.01);与OVX组相比,QCL组、EV组S-Ca、S-P、E2、PINP及骨密度水平升高(P<0.01),ALP、CTX-I、DPD水平降低(P<0.01,P<0.05);与EV组相比,QCL组S-Ca水平升高,PINP水平降低,DPD水平升高(P<0.01)。与Sham组相比,OVX组骨小梁结构松散不规则,骨髓腔变大;与OVX组相比,EV组和QCL组骨组织形态得到改善。结论黄瓜籽复合物能有效预防骨质疏松症,可能与提高E2、S-Ca、S-P水平,降低骨转换有关。

Objective To study the effect of cucumber seed compound on the prevention and treatment of osteoporosis in rats.Methods 24 female rats were randomly divided into sham operation group(Sham group),model group(OVX group),estradiol(E2)group(EV group)and cucumber seed compound group(QCL group).Bilateral oophorectomy was performed to establish the osteoporosis model.After 12 weeks of intervention by intragastric administration,serum calcium(S-Ca)and serum phosphorus(S-P)levels were detected by automatic biochemical analyzer;E2,alkaline phosphatase(ALP),collagen I amino terminal peptide(PINP),collagen I carboxyl terminal peptide(CTX-I)and deoxypyridinoline(DPD)levels were detected by ELISA;bone mineral density was analyzed with Micro-CT;the morphology of femur was observed by HE staining.Results Compared with the Sham group,the levels of S-Ca,S-P,E2,PINP and bone mineral density in the OVX group decreased(P<0.01),the levels of ALP,CTX-I and DPD increased(P<0.01).Compared with the OVX group,the levels of S-Ca,S-P,E2,PINP and bone mineral density in the QCL group and EV group increased(P<0.01),the levels of ALP,CTX-I and DPD decreased(P<0.01,P<0.05).Compared with the EV group,the S-Ca level increased,the PINP level decreased and the DPD level increased in the QCL group(P<0.01).Compared with the Sham group,the trabecular bone structure of the OVX group was loose and irregular,the bone marrow cavity became larger. Compared with the OVX group, the morphology of the EV group and the QCL group have been significantly improved. Conclusion Cucumber seed compound can effectively prevent osteoporosis, which may be related to up-regulate E2, S-Ca, S-P levels and down-regulate bone turnover.