甲基苯丙胺依赖者强制隔离康复戒断期Th/Treg细胞表达水平变化分析及其意义

Changes of Th and Treg Cells in Patients With Methamphetamine Dependence During the Period of Compulsory Isolation and Rehabilitation

目的:探讨甲基苯丙胺(methamphetamine,METH)依赖患者在强制隔离康复戒断期间的外周血CD4^(+)T细胞、Th1、Th2、Th17及Treg细胞的表达水平变化情况。方法:选取河北省强制隔离戒毒所2019年12月前收治的METH依赖初治患者30例,强制隔离康复戒断期6个月、戒断期12个月以及戒断期20个月患者各35例为病例组,同期健康体检者30例作为对照组。采用流式细胞术检测外周血单个核细胞中CD4+T细胞、Th1、Th2、Th17及Treg细胞表达水平。结果:METH依赖患者初治组外周血中CD4^(+)T细胞、Th1、Th17细胞比例、Th1/Th2及Th17/Treg比值均低于正常对照组,Th2及Treg细胞比例明显高于正常对照组,差异均有统计学意义(P<0.05)。METH依赖患者在强制隔离康复戒断0个月至第20个月期间,其外周血CD4^(+)T细胞与Th1细胞呈不断上升趋势,Th2与Treg细胞呈不断下降趋势,而Th17始终处于较低水平。结论:本次研究表明随着METH依赖患者康复戒断期限的延长,在戒断期间监测其CD4^(+)T细胞、Th1、Th2、Th17及Treg细胞表达水平的变化,可能有利于了解该类患者机体的免疫功能状况,为强制隔离戒毒康复医务工作者提供一些有用的新诊断评估指标。

Objective:To analyze the expression levels of CD4^(+)T lymphocyte,Th1,Th2,Th17 and Treg cells in peripheral blood of patients with methamphetamine dependence during the period of compulsory isolation and rehabilitation withdrawal.Methods:Thirty newly diagnosed patients with methamphetamine dependence admitted to our hospital before December 2019,35 patients with compulsory isolation and withdrawal for 6 months,12 months and 20 months were selected as the case group,and 30 healthy persons in the same period were selected as the control group.The expression levels of Th1,Th2,Th17 and Treg cells in peripheral blood mononuclear cells were detected by flow cytometry.Results:The proportion of CD4^(+)T cells,Th1,Th17 cells,Th1/Th2 and Th17/Treg ratio in peripheral blood of patients with METH dependence were lower than those in normal control group,and the ratio of Th2 and Treg cells was significantly higher than those of normal control group(P<0.05).During the period from 0 months to 20 months,CD4^(+)T cells and Th1 cells in peripheral blood of patients with METH dependence showed an increasing trend,Th2 and Treg cells showed a decreasing trend,while Th17 was always at a low level.Conclusion:This study shows that with the extension of the rehabilitation and withdrawal period of methamphetamine dependent patients,monitoring the expression levels of CD4^(+)T cells,Th1,Th2,Th17 and Treg cells during the withdrawal period may be helpful to understand the immune status of those patients,and provide some useful new diagnostic and evaluation indicators for medical workers in compulsory isolation and rehabilitation.