双向发酵提取燕麦β-葡聚糖的护肤功效研究

Study on the skin care effect of oatβ-glucan extracted by bidirectional fermentation

以燕麦作为培养基,通过双向发酵法提取燕麦中的β-葡聚糖,进行化妆品护肤功效研究。选用黄伞、大杯伞和灰树花3种真菌与燕麦进行双向发酵,实验结果表明,燕麦发酵后细胞毒性明显的降低。通过对成纤维细胞内活性氧(ROS)含量、Ⅰ型胶原蛋白(ColⅠ)含量及基质金属蛋白酶(MMP-1)含量进行测定发现,与发酵前相比,发酵液及纯化燕麦β-葡聚糖所作用的成纤维细胞内活性氧含量相对降低,Col I含量相对升高,MMP-1含量相对降低,证明发酵液具有一定的抗衰老功效,且比未经发酵处理的燕麦效果要好。通过对小鼠黑素瘤细胞(B16细胞)内黑色素含量及酪氨酸酶激活率的测定发现,发酵液处理后的细胞内黑色素含量更低,其中黄伞燕麦发酵液的效果最好。Franz扩散池透皮吸收实验证明发酵液的累积透过量更高。

Using oats as a culture medium,theβ-glucan in oats was extracted by a bidirectional fermentation method,and its efficacy of cosmetic skin care was studied.Three fungi,i.e.,Pholiota adiposa,Clitocybe maxima and Grifola frondose,were used for bidirectional fermentation with oats.The experimental results showed that the cytotoxicity of oats is significantly reduced after fermentation.By measuring the content of reactive oxygen species(ROS),type I collagen(Col I)and matrix metalloproteinase(MMP-1)in fibroblasts,it was found that the fermentation broth had certain anti-aging effect,and the effect was better than that of unfermented oats.Compared with those before fermentation,the fermentation broth and purified fermented oatβ-glucan showed relatively reduced content of active oxygen,relatively high content of Col I,and relatively low content of MMP-1 in fibroblasts.By measuring the melanin content and tyrosinase activation rate in mouse melanoma cells(B16 cells),it was found that the melanin content in the cells after being treated with the fermentation broth was lower,and the Pholiota adiposa/oat fermentation broth had the best effect.Franz diffusion cell transdermal absorption experiments had showed that the cumulative permeation amount of the fermentation broth is higher.