防晒乳液中8种紫外线吸收剂的UPLC-Q-TOF/MS定性及稳定性研究

UPLC-Q-TOF/MS qualitative analysis method and stability study of 8 ultraviolet absorbers in sunscreen cosmetic emulsions

基于45℃、75%RH的恒温恒湿加速试验,采用UPLC-Q-TOF/MS对不同类型的加速防晒乳液中的8种紫外线吸收剂进行了定性及稳定性研究。结果表明,在防晒乳液体系中,8种紫外线吸收剂的化学稳定性较差,与加速时间呈正相关降解,且采用UPLC-Q-TOF/MS分析时计算所得降解率明显高于HPLC分析。对紫外线吸收剂进行降解产物分析时发现,其某些降解产物可能与原型化合物具有相同的母核结构,故具有类似的紫外吸收特性和色谱保留行为,从而造成UPLC-Q-TOF/MS和HPLC分析时的结果差异。因此,相较于HPLC而言,UPLC-Q-TOF/MS灵敏度更高、专属性更强,可利用化合物特有的精确分子量,对结构和性质类似的不同降解程度的紫外线吸收剂降解产物实现更好地区分,更适用于紫外线吸收剂的稳定性研究。

Based on the constant temperature and humidity acceleration test at 45℃and 75%RH,UPLC-QTOF/MS was adopted to qualitatively study the stability conditions of 8 ultraviolet absorbers in different sunscreen cosmetic emulsions.The results show that the 8 ultraviolet absorbers are in poor chemical stability with a certain degree of degradation when they are added in the cosmetic emulsions,and the degradation rate under UPLC-QTOF/MS is significantly higher than that of HPLC.Analysis of possible degradation products revealed that some degradation products of UV absorbers may have the same nucleus structure as the prototype,which directly lead to the similar UV absorption characteristics and chromatographic retention behaviors.So,compared with HPLC,the UPLC-Q-TOF/MS has higher sensitivity and stronger specificity,and is able to achieve better differentiation of similar compounds by the unique precise molecular weight.Therefore,it is more suitable for the stability research of ultraviolet absorbers.