Influence of glucagon-like peptide 1 on the expression of ACAT1,CD36 gene and cholesterol metabolism in macrophages

Objective:To investigate whether glucagon like peptide-1(GLP-1)can reduce the cholesterol in foam cells derived from macrophages by affecting the expression of CD36 and acyl-CoA1(ACAT1)The content.Methods:Macrophages were obtained from mice,and after corresponding treatment,they were divided into 4 groups:blank control group,experimental group,GLP-1 agitation group,GLP-1 inhibition group,and the expression of CD36,ACAT1 mRNA and protein in cells of each group was detected.Expression and levels of total cholesterol(TC),free cholesterol(FC)and cholesterol ester(CE).Results:Compared with the blank control group,the content of TC,FC,CE and the expression of CD36,ACAT1 mRNA and protein in the experimental group were significantly increased(P<0.05);compared with the experimental group,the content and content of TC,FC,CE in the GLP-1 excited group The expressions of CD36 and ACAT1 mRNA and protein were significantly decreased(P<0.05);compared with the GLP-1 agitation group,the contents of TC,FC and CE and the expression of CD36,ACAT1 mRNA and protein were significantly increased in the GLP-1 inhibition group(P<0.05).Conclusion:The expression levels of CD36 and ACAT1 can be suppressed by GLP-1,and under the indirect influence of GPL-1,the level of intracellular cholesterol will also be reduced,so that the process of macrophages to foam cells is suppressed Thereby slowing down the development of atherosclerosis.