静电喷雾干燥微囊化乳双歧杆菌BL03

Electrostatic spray drying microencapsulated Bifidobacterium lactis BL03

静电喷雾干燥是新型微囊化益生菌的方法。该文研究了静电喷雾干燥乳双歧杆菌BL03保护剂和微囊化工艺,通过单因素和正交试验,优化得到保护剂组成为海藻糖14%,亚麻酸10%,CaHPO 46%,β-环糊精11%(均为质量分数),最佳静电喷雾微囊化工艺进风温度80℃,物料泵流量30 r/min,静电压25 kV,存活率高达89.26%。乳双歧杆菌BL03菌粉扫描电镜图片显示,静电喷雾干燥微囊化包埋效果最好,包埋率高达93.3%。比较静电喷雾干燥、常规干燥和乳化冷冻干燥3种微囊化方法,静电喷雾干燥模拟胃液作用2 h,存活率超过80%;模拟肠液作用4 h,存活率达到75%;25℃下储存12个月,活菌数存活率超过70%,均明显优于常规干燥和乳化冷冻干燥,说明静电喷雾干燥微囊化方法适合包埋乳双歧杆菌。

Electrostatic spray drying is a new microencapsulated probiotics method.The protectants and process of electrostatic spray drying were determined using single factor design and orthogonal experiment for Bifidobacterium lactis BL03.The optimized protectants were 14%trehalose,10%linolenic acid,6%calcium hydrophosphate and 11%β-cyclodextrin.The optimized process for electrostatic spray drying microencapsules were as follows:inlet temperature was 80℃,pump flow was 30 r/min,electrostatic charge was 25 kV and the survival rate was 89.26%.Scanning electron microscope(SEM)images showed that electrostatic spray drying had the best effect of microencapsulation.Embedding rate of microencapsules was 93.3%for B.lactis BL03.Compared electrostatic spray drying with traditional spray drying and freeze drying,the survival rate of electrostatic spray drying was above 80%when treated with simulated gastric fluid for 2 hours,and reached 75%with simulated intestinal fluid after 4 hours.The survival rate was over 70%at the temperature of 25℃after 12 months.Electrostatic spray drying was superior to traditional spray drying and freeze drying.