GPC1基因调节结直肠癌进展及预后的生物信息学分析

Bioinformatics analysis of GPC1 regulating the progression and prognosis of colorectal cancer

目的:利用生物信息学分析方法,分析GPC1基因在结直肠癌进展及预后中的作用,并预测可能的分子机制。方法:从TCGA数据库下载COAD转录数据及临床病理信息,通过Perl软件提取基因表达量,使用GSEA对基因集进行糖酵解相关通路富集及差异基因筛选。通过R软件limma包对差异基因进行单因素及多因素COX分析,筛选出6个相关基因并验证。然后分析6个相关基因的表达水平与COAD不同临床病理特征的相关性,其中GPC1基因具有相关性。最后通过R软件进行KEGG富集分析及相关靶基因相关性分析,预测目标基因调控结直肠癌进展预后的可能分子机制。结果:GPC1基因在COAD组织中高表达(P<0.001),与肿瘤的分级、TNM分期具有相关性(P<0.05)。皮尔森检验显示,GPC1与TGF-β1,VEGFB呈中等强度相关。结论:GPC1可能通过增强TGF信号通路和VEGF信号通路影响结直肠癌细胞的增殖、侵袭及结直肠癌的预后。

Objective:To analyze the role of gene GPC1 in the progression and prognosis of colorectal cancer by bioinfor⁃matics method to further predict possible molecular mechanisms.Methods:The colorectal cancer gene expression data and clinical data were downloaded from the TCGA database.The data were analyzed by Perl and R software,and COADrelated data were correlated with the glycolysis process using gene set enrichment analysis(GSEA)to determine the gly⁃colysis-related genes that regulate COAD.Using COX regression analysis,glycolysis-related genes associated with the prognosis of COAD were identified.The prognosis-related genes were screened to further analyze the survival and clinico⁃pathological correlation.GPC1 was identified as the target gene.The relationship beteween GPC1 and different clinical pathology,and patient survival were analyzed.Finally,GSEA was used to analyze GPC1 KEGG pathway and the correla⁃tion between GPC1 and potential target gene was analyzed with R software.We predicted the probable mechanisms of the target gene in regulating the progression and prognosis of colorectal cancer.Results:GPC1 was highly expressed in CO⁃AD tissues(P<0.001),and correlated with the grade and TNM stage of COAD(P<0.05).GSEA showed GPC1 was en⁃riched in TGF-β1 and VEGF signaling pathway.Conclusion:GPC1 regulates the proliferation,migration and prognosis of colorectal cancer through the TGF and VEGF signaling pathway.