LINC01515通过调控Ki67/p21蛋白的表达影响非小细胞肺癌细胞增殖与凋亡

LINC01515 is Associated With the Proliferation and Apoptosis of Non⁃Small Cell Lung Adenocarcinoma by Regulating the Expression of Ki67/p21 Protein

目的研究长链非编码RNA(long non⁃coding RNA,lncRNA)LINC01515对非小细胞肺腺癌细胞增殖、凋亡的影响。方法采用qRT⁃PCR法检测4株非小细胞肺腺癌细胞和1株正常支气管上皮细胞LINC01515的表达水平,选择LINC01515表达水平最高的H1975和H838两株细胞进行后续实验。将siLINC01515转染细胞后,qRT⁃PCR检测转染效率,并用WST法检测各细胞增殖活力,流式细胞术检测细胞凋亡,蛋白质免疫印迹(western blot,WB)法检测细胞中Ki67、p21表达水平。结果在H1975、PC⁃9、H838、H1650和HBE五种细胞系中,H1975和H838细胞LINC01515表达水平最高。与对照组比较,siLINC01515组细胞增殖活力显著降低(P<0.01)、凋亡期细胞比例显著升高(P<0.01),Ki67表达水平显著降低,p21表达水平显著升高(均P<0.01)。结论敲低LINC01515通过抑制细胞增殖相关蛋白及促进凋亡相关蛋白的表达,进而影响非小细胞肺腺癌细胞的增殖和凋亡。

Objective To study the effects of long non⁃coding RNA(lncRNA)LINC01515 on the proliferation and apoptosis of non⁃small cell lung adenocarcinoma cells.Methods The expression levels of LINC01515 in 4 kinds of non⁃small cell lung adenocarcinoma cells and 1 kind of normal bronchial epithelial cell were detected by qRT⁃PCR,and the two cell lines with the highest expression levels,H1975 and H838,were selected for subsequent experiments.After transfecting the cells with siLINC01515,we detected the transfection efficiency by qRT⁃PCR,detected the proliferation activity of each cell lines by WST method,detected apoptosis by flow cytometry,and detected Ki67 and p21 expression levels in the cells by western blot.Results Among the 5 cell lines of H1975,PC⁃9,H838,H1650 and HBE,H1975 and H838 with the highest expression level of LINC01515 were selected as the research objects.Compared with the control group,the cell proliferation activity of the siLINC01515 group was significantly reduced(P<0.01),the proportion of cells in the apoptotic phase was significantly increased(P<0.01),the expression of Ki67 was significantly reduced,and the expression of p21 was significantly increased(both P<0.01).Conclusions Knockdown of LINC01515 could suppress the proliferation⁃related protein expression and promote the apoptosis⁃related protein expression,thereby affecting the proliferation and apoptosis of non⁃small cell lung adenocarcinoma cells。