Clinical significance of lncRNA LINC01410 in preeclampsia and its effect on trophoblast proliferation and invasion

Objective:To study the expression of lncRNA LINC01410 in preeclampsia and normal placenta,and to explore its function and mechanism of affecting the proliferation and migration of trophoblast cells.Methods:The expression level of LINC01410 in the placenta of preeclampsia patients and pregnant women with normal pregnancy was detected by qRTPCR.After silencing LINC01410 in trophoblast cells HTR-8/Svneo,CCK-8 measured cell proliferation,and Transwell detected trophoblast invasion.Nucleus and chromatin RNA scores were measured,and Fas was detected by qRT-PCR and Western blot to explore the possible mechanisms.Results:The relative expression of LINC01410 in the placenta of the preeclampsia group was 2.564±0.169,which was significantly higher than 0.821±0.052 in the placenta tissue of the normal control group.After silencing LINC01410,the proliferation of trophoblast HTR-8/Svneo slowed down and the invasion ability decreased;The expression in the nucleus is more than in the cytoplasm,and the expression of Fas mRNA and protein is significantly reduced after silencing LINC01410.Conclusion:The high expression of lncRNA LINC01410 in preeclampsia placenta may be a risk factor for preeclampsia,and its mechanism may be to promote the proliferation and invasion of trophoblast cells by upregulating Fas expression.