黄连对高脂饮食诱导肥胖小鼠胰岛保护作用的实验研究

Protective Effect of Huanglian(Coptidis Rhizomaon)on Islets of High Fat Diet-Induced Obese Mice

目的:观察黄连对高脂饮食诱导的肥胖小鼠胰岛的保护作用。方法:采用C57BL/6小鼠,高脂饮食建立肥胖模型。随机分成模型组,黄连组,另设正常对照组,每组7只。黄连组给予黄连水煎剂灌胃4周,实验末,检测空腹血糖(FBG)、血清胰岛素(INS)。HE染色对胰腺组织进行病理学观察,免疫组织化学检测胰岛insulin蛋白表达。结果:与模型组比较,黄连组FBG、INS显著降低,与正常组相比没有差异;HE染色观察发现模型组较正常对照组胰岛数量增多,体积显著增大,黄连组与模型组相比胰岛数量减少,体积明显缩小,未见明显的毛细血管扩张充血;免疫组织化学法检测发现黄连组与模型组相比胰岛insulin蛋白表达明显减少。结论:黄连对高脂饮食诱导的肥胖小鼠胰岛有一定的保护作用。

Objective:To observe the protective effect of Coptidis Rhizoma on islet morphology of high-fat diet-induced obese mice.Methods:C57BL/6 mice were fed high-fat diet to establish obese model.They were randomly divided into model group and Coptidis Rhizoma group and normal diet group with each 7 mice.Coptidis Rhizoma group was given Huanglian decoction by gavage for 4 weeks.At the end of the experiment,fasting blood glucose(FBG)and serum insulin(INS)were measured.HE staining was used for pathological examination of the pancreatic islet tissue,and the insulin protein expression was detected by Immunohistochemistry(IHC).Results:Compared with the model group,FBG and INS in Coptidis Rhizoma group decreased significantly,and there was no difference compared with the normal group.HE staining showed that the number and volume of islets in Coptidis Rhizoma group decreased significantly,and no obvious telangiectasia and congestion were observed.Immunohistochemical results suggested that the insulin protein expression decreased significantly.Conclusion:Coptidis Rhizoma has a protective effect on islets of obese mice induced by high fat diet.