肿瘤坏死因子的调节性T细胞与肝癌发生的相关性

Correlation between Tumor Necrosis Factor and Regulatory T Cells in Hepatocellular Carcinoma

目的探讨肿瘤坏死因子(tumor necrosis factor,TNF)的调节性T细胞与肝癌发生的相关性。方法选取124例肝癌患者作为肝癌组,选择同期健康查体者124例作为对照组。检测2组血清TNF-α含量与肝功能,采用流式细胞仪检测外周血CD4^(+)调节性T细胞比例并进行相关性分析。结果肝癌组的血清TNF-α含量高于对照组,血清AFP、ALT、AST、TBil含量也高于对照组,对比差异都有统计学意义(P>0.05)。肝癌组的CD4^(+)调节性T细胞比例显著低于对照组(P<0.05)。相关分析显示肝癌患者CD4^(+)调节性T细胞比例与TNF-α、AFP、ALT、AST、TBil都存在相关性(P<0.05)。多元线性回归分析显示TNF-α、AFP、ALT为影响CD4^(+)调节性T细胞比例的主要因素(P<0.05)。结论肝癌患者的外周血CD4^(+)调节性T细胞比例呈现下降趋势,与患者的TNF-α含量与肝功能状态有相关性。

Objective To explore the correlation between tumor necrosis factor(TNF)and regulatory T cells in hepatocellular carcinoma.Methods 124 patients with liver cancer were selected as the liver cancer group,and 124 cases of healthy patients were selected as the control group at the same period.The erum TNF-αlevels and liver function were detected in the two groups,and the proportion of CD4^(+)regulatory T cells in peripheral blood were detected by flow cytometry and given correlation analysis.Results The content of serum TNF-αin the hepatocellular carcinoma group were higher than that in the control group,and the content of serum AFP,ALT,AST,and TBil were also higher than that in the control group,and compared the differences were statistically significant(P>0.05).The proportion of CD4^(+)regulatory T cells in the hepatocellular carcinoma group were significantly lower than that in the control group(P<0.05).In the hepatocellular carcinoma group,Spearman correlation analysis showed that the proportion of CD4^(+)regulatory T cells were correlated with TNF-α,AFP,ALT,AST,and TBil(P<0.05).Multiple linear regression analysis showed that TNF-α,AFP and ALT were the main factors affectedthe proportion of CD4^(+)regulatory T cells(P<0.05).Conclusion The percentage of CD4^(+)regulatory T cells in peripheral blood of patients with hepatocellular carcinoma shows downward trend,which are related to the TNF-αcontent of patients and liver function status.