宫颈癌患者HPV感染状况及外周血Th1/Th2细胞因子变化研究

HPV Infection Status,Changes of Th1 and Th2 in Peripheral Blood of Uterine Cerivical Cancer Patients

目的探讨宫颈癌患者人乳头瘤病毒(HPV)感染状况及外周血Th1/Th2细胞因子变化,分析其在宫颈癌发生及发展中所起的可能机制,为宫颈癌的临床防治提供参考。方法选取80例宫颈癌患者为研究对象(宫颈癌组),选取同期门诊体检的健康女性80例为对照(对照组),采用荧光定量聚合酶链反应(FQ-PCR)检测宫颈HPV-DNA,应用酶联免疫吸附法检测Th1/Th2细胞因子水平,包括白细胞介素-2(IL-2)和干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)和白细胞介素-6(IL-6)。结果宫颈癌组70例检出HPV,感染率为87.50%,对照组5例检出HPV,感染率为6.25%,比较差异有统计学意义(P<0.05),宫颈癌组HPV分型60例高危型、10例低危型,分别占85.71%、14.29%,38例单一感染、32例多重感染,分别占54.29%、45.71%,对照组HPV分型0例高危型、5例低危型,分别占0.00%、100.00%,5例单一感染、0例多重感染,分别占100.00%、0.00%,两组HPV分型及感染类型比较差异有统计学意义(P<0.05);宫颈癌组外周血IL-2和IFN-γ、IL-4、IL-6水平分别为(43.08±8.56)pg/ml、(56.15±9.47)pg/ml、(28.55±6.39)pg/ml、(34.29±7.80)pg/ml,明显高于对照组(18.94±4.57)pg/ml、(28.28±7.78)pg/ml、(12.39±3.90)pg/ml、(16.62±3.49)pg/ml,比较差异有统计学意义(P<0.05);宫颈癌组HPV感染患者外周血IL-2和IFN-γ、IL-4、IL-6水平明显高于无HPV感染患者,比较差异有统计学意义(P<0.05);对照组HPV感染者外周血IL-2和IFN-γ、IL-4、IL-6水平明显高于无HPV感染者,比较差异有统计学意义(P<0.05)。结论宫颈癌患者存在HPV感染率增高及外周血Th1/Th2细胞因子紊乱,HPV感染可加重Th1/Th2细胞因子紊乱,HPV感染及外周血Th1/Th2细胞因子紊乱在宫颈癌的发生发展中可能起到了促进作用。

Objective To discuss the HPV infection status,changes of Th1 and Th2 in peripheral blood of uterine cerivical cancer patients,to analyze the possible mechanism of uterine cerivical cancer in its occurrence and development,it provides reference for the clinical prevention and treatment of cuterine cerivical cancer.Methods The 80 cases uterine cerivical cancer patients(uterine cerivical cancer group),according to the actual timing of tracheotomy,and 90 healthy women were included in the analysis(control group),fluorescence quantitative polymerase chain reaction(FQ-PCR)was used to detect cervical HPV-DNA,the levels of Th1/Th2 cytokines were detected by enzyme-linked immunosorbent assay,these include interleukin-2(IL-2)and interferon-γ(IFN-γ),interleukin-4(IL-4),and interleukin-6(IL-6).Results 70 cases of HPV infection of the uterine cerivical cancer group,the infection rate were 87.50%,5 cases of HPV infection of the control group,the infection rate were 6.25%,the difference was statistically significant(P<0.05);There were 60 cases of high-risk type(85.71%)and 10 cases of low-risk type(14.29%),38 cases of single infection(54.29%)and 32 cases of multiple infection(45.71%)with the uterine cerivical cancer group,there were 0 case of high-risk type(0.00%)and 5 cases of low-risk type(100.00%),5 cases of single infection(100.00%)and 0 case of multiple infection(0.00%)with the control group,the difference wasstatistically significant(P<0.05).IL-2 were(43.08±8.56)pg/ml,IFN-γwere(56.15±9.47)pg/ml,IL-4 were(28.55±6.39)pg/ml,IL-6 were(34.29±7.80)pg/ml of the uterine cerivical cancer group,IL-2 were(18.94±4.57)pg/mlpg/ml,IFN-γwere(28.28±7.78)pg/ml,IL-4 were(12.39±3.90)pg/ml,IL-6 were(16.62±3.49)pg/ml of the control group,the difference wasstatistically significant(P<0.05).IL-2,IFN-γ,IL-4,IL-6 of HPV infection patients higher than non HPV infection patients higher the uterine cerivical cancer group,IL-2,IFN-γ,IL-4,IL-6 of HPV infection patients higher than non HPV infection patients higher the control group,the difference wasstatistically significant(P<0.05).Conclusion HPV infection higher,inordinate of Th1 and Th2 in peripheral blood of uterine cerivical cancer patients,HPV infection and Th1/Th2 cytokine disorder in peripheral blood may play a role in promoting the development of cervical cancer.