木贼镰孢基因功能注释及比较基因组学分析

Genes Functional Annotation and Comparative Genomic Analysis of Fusarium equiseti

为挖掘木贼镰孢(Fusarium equiseti(Corda)Sacc.)的产毒基因及明确其进化关系,通过BLAST软件与GO、KEGG、COG、EggNOG、CAZy等14个数据库结合的方法对其全基因组进行功能注释并挖掘产毒基因,进行系统进化分析及运用色谱技术研究产毒基因的分泌规律;以麦根腐平脐蠕孢、燕麦镰孢、尖孢镰孢、假禾谷镰孢、茄病镰孢的全基因组序列为参考序列进行比较基因组学分析并确定其系统进化关系。结果发现,木贼镰孢的功能基因达13134个,占总全基因组的94.97%,并有多个与木贼镰孢细胞过程、新陈代谢、分子功能、致病性相关的基因,挖掘到2个与玉米赤霉烯酮合成相关的产毒基因ALM1和ALM2;且木贼镰孢在液体培养至第5~8天时,分泌玉米赤霉烯酮的速率最快。比较基因组学分析发现,木贼镰孢与参考菌株共有基因7378个,与假禾谷镰孢的遗传距离最近。研究结果为进一步基因调控机制的研究及病害防控提供参考。

In order to tap toxin-producing genes and identify its evolutionary relationships of Fusarium equiseti,Basic Local Alignment Search Tool(BLAST)and 14 databases GO,KEGG,COG,EggNOG,and CAZy were adopted to functionally annotate its whole genome and toxin-producing gene,the excretion law of toxin producing gene was studied conducting systematic analysis and applying chromatography;the whole genome sequences of Bipolaris sorokiniana,F.avenaceum,F.oxysporum,F.graminearum,and F.solani,were taken as reference sequences and conducted comparison genomics analysis to decide its systemic evelutionary relationship.The results suggested that F.equiseti had as many as 13134 functional genes,accounted for 94.97%of the whole genome,and there were many correlative genes to cellular process,metabolism,molecular function,and pathogenicity,and excavated two toxin-producing genes related to zearalenone synthesis ALM1 and ALM2;and F.equiseti secreted zearalenone the fastest from 5th to 8th days in its cultivation in liquid.Comparison genomic analysis found that there were 7378 common genes in F.equiseti to those of the reference strains,and the genetic distance between F.equiseti and F.graminearum was the shortest.This research results provided an effective theoretical basis for further study on gene regulation mechanism and plant disease prevention and control.