蒲公英多酚提取工艺优化及抗氧化活性研究

Study on Optimization of Extraction Technology and Antioxidant Activity of Dandelion Polyphenols

为研究蒲公英多酚的利用价值以及后续的开发,采用超声波微波提取蒲公英超微粉中多酚,在单因素的基础上,通过Box-Behnken和响应面法对提取多酚的工艺条件(包括乙醇体积分数、料液比、微波时间、超声时间、超声功率、微波功率、提取温度)进行优化,确定最佳提取工艺为乙醇体积分数50%、料液比1∶45、微波时间3 min、超声时间60 min、超声功率240 W、微波功率350 W、提取温度50℃,在上述条件下,多酚提取率是2.96%。结果表明,蒲公英多酚具有清除DPPH自由基、ABTS自由基的能力,并对铁的还原也有一定的作用。

In order to study the utilization value of dandelion polyphenols and subsequent development,ultrasonic microwave was used to extract polyphenols from dandelion ultrafine powder.On the basis of single factor,the process conditions for extracting polyphenols(including ethanol volume fraction,solid-to-liquid ratio,microwave time,ultrasonic time,ultrasonic power,microwave power,extraction temperature)were optimized by Box-Behnken and response surface method.The optimal extraction process was determined to be 50%ethanol volume fraction,1∶45 solid-to-liquid ratio,microwave time 3 min,ultrasonic time 60 min,ultrasonic power 240 W,microwave power 350 W,extraction temperature 50℃.Under the above conditions,the extraction rate of polyphenols was 2.96%.The results showed that dandelion polyphenols had the ability to scavenge DPPH free radicals and ABTS free radicals,and also had a certain effect on iron reduction.