茶多酚通过调控XB130对LPS诱导小鼠肺泡巨噬细胞炎症因子表达的影响

Effect of Tea Polyphenols on LPS Induced Inflammatory Factor Expression in Mouse Alveolar Macrophages by Regulating XB130

目的:研究茶多酚(TP)对肺炎小鼠肺泡巨噬细胞炎症因子表达的影响及潜在作用机制。方法:用脂多糖(LPS)诱导小鼠肺泡巨噬细胞MH-S 12h建立小鼠肺泡巨噬细胞炎症模型。将MH-S细胞分为对照组(LPS处理)和TP组(LPS+TP处理)、pcDNA3.1-NC组(转染pcDNA3.1-NC+LPS处理)、pcDNA3.1-XB130组(转染pcDNA3.1-XB130+LPS处理)、si-NC组(转染si-NC+LPS处理)、si-XB130组(转染si-XB130+LPS处理)、TP+si-NC组(转染si-NC+LPS+TP处理)和TP+si-XB130组(转染si-XB130组+LPS+TP处理),qRT-PCR检测XB130 mRNA表达,Western blot检测XB130表达以及白细胞介素6(IL-6)、IL-1β和肿瘤坏死因子α(TNF-α)分泌。结果:与对照组相比,TP组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著降低(P<0.05),XB130 mRNA和蛋白表达显著升高(P<0.05)。与pcDNA3.1-NC组比较,pcDNA3.1-XB130组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著降低,XB130蛋白表达显著升高(P<0.05)。与si-NC组比较,si-XB130组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著升高,XB130蛋白表达显著降低(P<0.05)。与TP+si-NC组比较,TP+si-XB130组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著升高,XB130 mRNA和蛋白表达显著降低(P<0.05)。结论:茶多酚可降低LPS诱导的小鼠肺泡巨噬细胞炎症因子表达水平,其机制与上调XB130表达有关。

Objective: To study the effect of tea polyphenols(TP) on the expression of inflammatory factors in alveolar macrophages of pneumonia mice and its potential mechanism. Methods: Mouse pneumonic cell model was established by inducing mouse alveolar macrophages MH-S with lipopolysaccharide(LPS) for 12 h. MH-S cells were divided into control(LPS treatment) group,TP group(LPS+TP treatment),pcDNA 3.1-NC group(pcDNA 3.1-NC+LPS treatment),pcDNA 3.1-XB130 group(pcDNA 3.1-XB130+LPS treatment),si-NC group(si-NC+LPS treatment),si-XB130 group(si-XB130+LPS treatment),TP +si-NC group(si-NC+LPS+TP treatment) and TP+si-XB130 group(si-XB130+LPS+TP treatment). qRT-PCR was used to detect the expression level of XB130 mRNA,and Western blot was applied to measure the expression level of XB130 and the secretions of interleukin 6(IL-6),IL-1β and tumor necrosis factor α(TNF-α) . Results: Compared with those in the control group,the secretions of IL-6,IL-1β and TNF-α in MH-S cells of TP group were significantly reduced(P<0.05),and the expressions of XB130 mRNA and protein were significantly increased(P<0.05). Compared with those in pcDNA3.1-NC group,the secretions of IL-6,IL-1β and TNF-α in MH-S cells of pcDNA3.1-XB130 group were significantly reduced,the expressions of XB130 mRNA and protein significantly increased(P<0.05). Compared with those in si-NC group,the secretions of IL-6,IL-1β and TNF-α in MH-S cells of si-XB130 group were significantly increased,the expressions of XB130 mRNA and protein significantly decreased(P<0.05). Compared with those in TP+si-NC group,the secretions of IL-6,IL-1β and TNF-α in MH-S cells of TP+si-XB130 group were significantly increased,the expressions of XB130 mRNA and protein significantly decreased(P<0.05). Conclusion: Tea polyphenols can reduce LPS-induced inflammatory cytokine expression levels in mouse alveolar macrophages,and its mechanism is related to the up-regulation of XB130 expression.