浓香型酒醅中一株耐热地衣芽孢杆菌产淀粉酶和蛋白酶培养条件的优化

Optimization of Conditions for Amylase and Protease Production of a Thermotolerant Bacillus Licheniformis Strain

以浓香型酒醅分离得到的一株地衣芽孢杆菌为试验菌株。以培养温度、培养时间、转速作为主要影响因素,通过单因素试验和正交试验对该菌株产淀粉酶和蛋白酶的条件进行优化,该菌株同时产淀粉酶和蛋白酶的最优条件为:培养温度40℃,培养时间36h,培养转速150r/min,此条件下,蛋白酶活力达到最高的51.84U/mL,淀粉酶活力达到37.36U/mL。

A Bacillus licheniformis isolated from luzhou flavour fermented grains was used as the experimental strain.The culture temperature,time and speed as the main influencing factors.The conditions of amylase and protease production were optimized by single factor test and orthogonal test.The optimum conditions for producing amylase and protease simultaneously was the culture temperature was 40℃,the culture time was 36h,and the culture speed was 150r/min,Under these conditions,The protease activity reached the highest 51.84U/mL and the amylase activity reached 37.36U/mL.